摘要
目的评价他克莫司联合窄谱中波紫外线(NB-UVB)对人表皮黑素细胞酪氨酸酶活性及黑素合成的影响。方法用MTT法筛选他克莫司最佳作用浓度,根据对黑素细胞不同干预方法分为:他克莫司组、NB-UVB组、他克莫司+NB-UVB组,未处理组为对照组。NB-UVB的照射剂量为25mJ/cm2,以左旋多巴为底物测定酪氨酸酶活性,NaOH法测定黑素合成的影响。结果他克莫司(10、102、103 nmol/L)对黑素细胞增殖无明显影响(P>0.05),104 nmol/L他克莫司明显抑制黑素细胞增殖(P<0.05),故选择103 nmol/L为最佳浓度用于后续实验;他克莫司(103 nmol/L)、NB-UVB(25mJ/cm2)、他克莫司+NB-UVB三组酪氨酸酶活性及黑素合成均明显高于对照组(P<0.05);他克莫司+NB-UVB组酪氨酸酶活性及黑素合成高于他克莫司组及NB-UVB组(P<0.05)。结论他克莫司联合NB-UVB可以协同增加黑素细胞酪氨酸酶活性及黑素合成。
Objective To evaluate the effects of narrow band-UVB （NB-UVB） combining tacrolimus on melanogenesis and tyrosinase activities in human melanocytes. Methods MTT assay was performed to select the optimal concentration of tacrolimus. Melanocytes were classified into tacrolimus group, NB-UVB group and NB- UVB combining tacrolimus group, respectively; those receiving no treatment served as controls. Tyrosinase activities were evaluated with L-dopa as the substrate. Melanin content was detected by NaOH assay. Results There was no significant difference in proliferation of melanocytes in tacrolimus group （10, 102, 103 nmol/L） compared with the control group （P 〈 0. 05）, and tacrolimus （104 nmol/L） decreased the proliferation of melanocytes markedly compared with the control group （P〈0.05）. Therefore, the optimal concentration was 103 nmol/L. Tyrosinase activities and melanin content increased significantly in tacrolimus group, NB-UVB group, and NB-UVB combining tacrolimus group compared with the control group （P 〈 0. 05）. Tyrosinase activities and melanin content increased more markedly in NB-UVB combining tacrolimus group than in tacrolimus group or NB- UVB group （P 〈 0. 05）. Conclusion Tacrolimus combining NB-UVB treatment can synergistically enhance activities of tyrosinase and accelerate melanogenesis.
出处
《西安交通大学学报（医学版）》
CAS
CSCD
北大核心
2013年第2期237-239,252,共4页
Journal of Xi’an Jiaotong University（Medical Sciences）
