摘要
目的观察胎盘组织中血管内皮生长因子受体-1（VEGFR-1）、VEGFR-2、血浆中可溶性血管内皮生长因子受体-1（sVEGFR-1）及sVEGFR-2的表达水平，并探讨其在子癎前期的临床检测意义。方法ELISA检测正常妊娠期妇女和子癎前期患者血浆中的sVEGFR-1和sVEGFR-2的表达；RT-PCR检测正常妊娠期妇女和子癎前期患者胎盘中VEGFR-1、VEGFR-2、sVEGFR-1和sVEGFR-2mRNA的表达。结果ELISA检测结果显示，正常妊娠组分娩后较分娩前血浆中sVEGFR-1[（4．55±0．31）ng／mlvs（12．33±1．52）ng／m1]水平降低，子癎前期组分娩后血浆中sVEGFR．1[（8．13±0．74）ng／mlvs（77．25±9．47）ng／m1]水平亦降低，两组分娩前后数据变化比较差异有统计学意义（P〈0．01）；正常妊娠组分娩前后血浆中sVEGFR-2分别为（8．74±1．24）ng／ml与（6．43±0．55）ng／ml，而子痫前期组分别为（5．69±0．75）ng／ml与（4．96±0．67）ng／ml；RT-PCR检测结果与其-致。结论子癎前期患者分娩后VEGFR-1血浆水平迅速下降，而VEGFR-2持续低水平表达，提示VEGFR-1可能与子癎前期发病率有关，而子癎前期患者血浆中VEGFR-2水平的降低可作为内皮功能障碍的标志。
Objective To analyze tissue vascular endothelial growth factor receptor 1 （VEGFR-1） and VEGFR-2 and their soluble form （ sVEFGR-1 and sVEGFR-2） in the plasma in patients with preeclampsia, and to explore its clinical significance. Methods sVEGFR-1 and sVEGFR-2 expressions in plasma of normal pregnancy women and preeclampsia patients were detected by enzyme-linked immunosorbnent assay （ELISA） ; VEGFR-1, VEGFR-2, sVEGFR-1 and sVEGFR-2 mRNA expressions of normal pregnancy women and preeclampsia patients in placenta were detected by reverse transcription-polymerase chain reaction （RT-PCR）. Results ELISA results showed that the level of sVEGFR-1 in plasma of normal control group before and after delivery was （ 12. 33 ± 1.52） ng/ml and （4. 55 ± 0. 31 ） ng/ml, respectively, while that of preeclampsia group was （ 77. 25 ± 9.47 ） ng/ml and （ 8.13 ± 0. 74） ng/ml, respectively; the differences between before and after delivery in the two groups were of statistical significancel The level of sVEGFR 2 in plasma of normal control group before and after delivery was （ 8.74 ± 1.24 ） ng/ml and （ 6. 43 ± 0. 55 ） ng/ml, respectively, while that of preeclampsia group was （ 5.69 ± 0.75 ） ng/ml and （4. 96 ± 0. 67） ng/ml, respectively. RT-PCR results were consistent with ELISA results. Conclusions Rapid decrease of plasma sVEGFR-1 and continuously low-level expression of plasma sVEGFR-2 indicated that VEGFR-1 might be closely related to preeclampsia, and decrease of plasma sVEGFR-2 in preeclampsia women might be taken as a marker of endothelial cell function disorder.
出处
《中国医师杂志》
CAS
2013年第1期52-55,共4页
Journal of Chinese Physician
