摘要
通过分析预处理前造血干细胞(hematopoietic stem cell,HSCs)的状态(数量、在细胞周期的分布、活性氧的状态、凋亡情况、DNA损伤程度、DNA损伤相关酶活性)和功能(体外的集落检测、NOD/SCID小鼠体内造血重建功能),提出用地贫患者HSCs评价其骨髓移植危险因素和等级,以及根据预处理过程中HSCs根除情况调整预处理方案是未来地贫骨髓移植发展的两大趋势.
Thalassemia is a common hematologic disorder inherited through single -gene mutation. At present, al- logeneic hematopoietic stem cells （HSCs） transplantation is the only rational therapeutic modality for the eradica- tion of thalassemia major. The promising strategies in bone marrow transplantation （BMT） of thalassemia major are （ 1 ） a method evaluating the risk factors and classes of BMT is established through analyzing the state （ the number of long- term HSCs, the percentage of quiescent HSCs and proliferative status of HSCs in the cell cycle, reactive oxygen species, apoptosis, oxidative DNA poietic reconstitution capacity of HSCs in ment; （2） preparatory regimen of BMT is transplant treatment. damage, DNA repair enzymes） and functions （ colony assay, the hemato- recipient mice of NOD/SCID） of HSCs before the pre - transplant treat- adjusted based on the elimination of HSCs during the process of pre -transplant treatment.
出处
《华南师范大学学报（自然科学版）》
CAS
北大核心
2013年第1期10-13,共4页
Journal of South China Normal University(Natural Science Edition)
基金
美国国家卫生研究院基金项目(R01 HL076712)
关键词
地中海贫血
骨髓移植
造血干细胞
预处理方案
危险等级
thalassemia
bone marrow transplantation
hematopoietic stem cell
preparatory regimens
risk class
