摘要
目的观察血管内皮生长因子(vascular endothelial growth factor,VEGF)及血小板衍生生长因子(plateletderived growth factor,PDGF)在骨折患者及合并脑外伤骨折患者血清中的含量变化,探讨VEGF、PDGF在骨折合并脑外伤后的骨折加速愈合中的作用机制,为临床上促进骨折愈合的细胞因子疗法寻找理论依据。方法选择单纯四肢长管状骨骨折患者20例,合并脑外伤四肢长管状骨骨折患者20例,分别于伤后第3天、第10天抽取静脉血,用酶联免疫吸附(enzyme-linked immunosorbent assay,ELISA)测定患者血清中VEGF、PDGF含量。结果 a)第3天,单纯骨折组、合并脑外伤骨折组患者血清中VEGF含量分别为(77.96±20.05)pg/mL和(134.79±20.39)pg/mL,P<0.05,有统计学意义。单纯骨折组、合并脑外伤骨折组患者血清中PDGF含量分别为(193.37±51.56)pg/mL和(248.01±94.14)pg/mL,P<0.05,有统计学意义。b)第10天,单纯骨折组、合并脑外伤骨折组患者血清中VEGF含量分别为(155.11±25.42)pg/mL和(210.63±24.63)pg/mL,P<0.05,有统计学意义。单纯骨折组、合并脑外伤骨折组患者血清中PDGF含量分别为(346.99±92.91)pg/mL和(486.20±88.09)pg/mL,P<0.05,有统计学意义。c)患者血清VEGF与PDGF含量呈正相关,r=0.885。结论脑外伤后血清VEGF、PDGF含量升高是加速骨折愈合的因素之一,且两者在骨折愈合过程中相互协同发挥作用。
Objective To investigate the serum vascular endothelial growth factor（VEGF） and platelet derived growth factor （PDGF） in the patients of simple fracture and fracture complicated with traumatic brain injury（TBI）. To explore the accelerated factors of fracture healing in the fracture complicated with TBI. Methods Venous blood samples of 20 patients of simple fracture and 20 patients of fracture complicated with TBI were collected in the third day and the the tenth day after fracture. Enzyme linked immunosorbent assay（ELISA）was used to detect the serum level of VEGF and PDGF. Results On the third day the serum concentration of VEGF in simple fracture and fracture complicated with TBI were（77.96±20.05） pg/mL and（134.79±20.39） pg/mL. There was significant difference between the two groups, （P〈0.05）. On the third day the serum concentration of PDGF in simple fracture and fracture complicated with TBI were （193. 374-51.56） pg/mL and （248.01±94. 14） μg/mL. There were significant difference in the two. Conclusion The rising of serum concentration of VEGF and PDGF after TBI is one of the factors to accelerate fracture healing. VEGF and PDGF are synergies in the process of fracture healing.
出处
《实用骨科杂志》
2013年第2期136-138,192,共4页
Journal of Practical Orthopaedics
关键词
骨折
脑外伤
血管内皮生长因子
血小板衍生生长因子
fracture
traumatic
brain injury
vascular endothelial growth factor
platelet derived growth factor
