摘要
目的:观察雷公藤内酯醇(triptolide,TPL)单独及联合硼替佐米(bortezomib,BZM)对激素抵抗性前列腺癌PC3细胞的体外生长和凋亡的影响。方法:选用递增浓度的TPL和BZM单独及联合作用于PC3细胞,MTT法检测PC3细胞生长抑制率;流式细胞仪检测PC3细胞凋亡;RT-PCR分析血管内皮生长因子(VEGF)mRNA的表达。结果:TPL、BZM单独对PC3细胞生长均有抑制作用且呈时间和剂量依赖性。相同浓度时,TPL单独作用强于BZM(P<0.05),两药联合可明显提高细胞生长抑制率(P<0.05);流式细胞检测发现,联合用药引起细胞凋亡也明显高于单独用药(P<0.05);TPL和BZM均能下调VEGFmRNA的表达,联合用药下调作用更加明显(P<0.05)。结论:不论是单独还是联合应用TPL和BZM均可抑制PC3细胞增殖并诱导其凋亡,两药联合作用明显强于单独用药,两者作用有协同性。
Objective To detect the effect of Castration-Resistant Prostate Cancer PC3 cells with triptolide（TPL） alone or combined with Bortezomib（BZM）. Methods MTF assay was performed to evaluate the growth in inhibitory rate of PC3 cells.PC3 cell apoptosis were determined by flow cytometry.RT-PCR was used to analyze the mRNA expressions of vascular endothelial growth factor （VEGF）. Results TPL and BZM both inhibited the growth of PC3 cells in a time-and-dose dependent manner, and the single drug of TPL was stronger than BZM （P 〈 0.05）in the same concentration.The combination group was stronger than the TPL or BZM alone （P 〈0.05）.From flow cytometry resuLts, the combination group was also stronger than the TPL or BZM alone （P 〈 0.05）. RT-PCR showed that TPL and BZM both down-regulated the mRNA expression of VEGF （P 〈 0.05）, combination was more obvious than the single drug. Conclusion TPL and BZM alone or combination inhibit the growth of PC3 cells and induce apoptosis .There is a synergistic effect when combination of TPL with BZM is in use.
出处
《中国中西医结合外科杂志》
CAS
2013年第1期46-49,共4页
Chinese Journal of Surgery of Integrated Traditional and Western Medicine
