摘要
采用NaOH水溶液对灰叶马尾藻(Sargassum cinereum)进行超声浸提,通过Sevag法脱蛋白、柱层析,分离得2种浅黄色海绵状水溶性马尾藻多糖SP1、SP2。在质量浓度2g/100mL NaOH水溶液中80℃超声1h,再在80℃恒温水浴4h,得最高多糖产率为13.4%。SP1、SP2苯酚-硫酸显色反应呈阳性,紫外扫描、电泳检测表明多糖纯度较好;凝胶渗透色谱测得SP1、SP2分子质量分别约为36、77kD,红外光谱显示SP1、SP2有硫酸基、羧基和α-D-半乳吡喃糖的特征吸收峰。以电子自旋共振技术研究SP1、SP2抗氧化活性,10mg/mL的SP1、SP2对羟自由基(OH)的清除能力分别为87.2%、61.5%;SP1、SP2均具有较强的抑制人前髓白细胞(HL60)生长的作用,而SP2抗癌活性更高。
Two yellow sponge polysaccharides （SP1 and SP2） were extracted from Sargassum cinereum after ultrasonic treatment in NaOH solution, protein removal through Sevag method, column separation, dialysis and lyophilization. Maximum yield of polysaccharide was up to 13.4% when the algae was subjected to ultrasonic extraction in 2% NaOH at 80℃ for 1 h and continuous treatment for 4 h in water bath. SP1 and SP2 revealed the positive phenol-sulfuric colorimetric reaction. UV and electrophoresis showed that SP1 and SP2 had high purity. According to GPC, the molecular weights of SP1 and SP2 were approximately 36 kD and 77 kD, respectively. Both SP1 and SP2 had characteristic FrlR absorption spectra of sulfate, carboxyl group and a-D- galactopyranosides. In addition, SP1 and SP2 showed good antioxidant activity. The hydroxyl radical scavenging capacity of SP1 and SP2 in aqueous solution （10 mg/mL） was 87.2% and 61.5%, respectively. Moreover, SP1 and SP2 also showed good ability to inhibit the proliferation of HL-60 cells. The anti-cancer activity of SP2 was higher than that of SP1.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2013年第3期93-96,共4页
Food Science
基金
海口市重点科技计划项目(2010-084
2011-0079)
海南大学"211工程"创新人才计划项目
关键词
灰叶马尾藻
多糖
抗癌活性
抗氧化活性
Sargassum cinereum
polysaccharide： anti-cancer activity
antioxidant activity
