摘要
目的:研究尼古丁体外对肺腺癌细胞生长及化疗敏感性的影响。方法:应用MTT探讨尼古丁对肺腺癌细胞SPC-A-1对5-FU,DDP和ADM化疗敏感性的影响;采用实时荧光定量PCR技术研究尼古丁对MDR1基因表达的影响。结果:1～100ng.mL-1尼古丁能显著促进SPC-A-1细胞的生长,各系列浓度的尼古丁(1,10,100,1000ng.mL-1)对SPC-A-1细胞的生长抑制率分别为-43.5%,-29.6%,-22.3%和28.0%。100ng.mL-1尼古丁分别与各系列浓度的5-FU(10～1000ng.mL-1)、DDP(50～50000ng.mL-1)和ADM(20～20000ng.mL-1)联合处理,均不提高SPC-A-1细胞的生长抑制率,且与对应各系列浓度的5-FU、DDP和ADM的单独处理相比,没有显著差异(P>0.05)。1～100ng.mL-1尼古丁处理后,在SPC-A-1细胞,未检测到MDR1基因表达;而在阳性对照细胞K562诱导MDR1基因表达。结论:尼古丁能显著促进SPC-A-1细胞生长,且能明显降低该细胞对1ng.mL-15-FU的化疗敏感性,但并不减低对DDP和ADM的化疗敏感性。尼古丁也不能显著诱导对SPC-A-1细胞的MDR1基因表达。
Objective：To investigate in vitro effect of nicotine on growth and chemosensitivity of lung adeno- carcinoma cells. Methods：MTT method was used to estimate chemosensitivity of lung adenoeareinoma cell line, SPC--A--1 to 5--Fu, DDP and ADM; and real--time quantification fluorescent PCR was used to examine the expression of MDR1 gene before and after nicotine exposure. Results：Nicotine was observed to significantly pro- mote growth of SPC--A--1 cell line at 1 to 100ng.mL：a. Growth inhibition rate of series concentration of nicotine（1, 10, 100 and 1000ng-mL-1） was --43.5%,--29.6%,--22. 3%, and 28. 0% in SPC--A--1 cells. No significant elevation of growth inhibition rates were observed in SPC--A--1 cells with combination of nicotine （100ng. mL-1 ） with series concentration of 5 -- FU （10- 1000ng.mL-1）, or DDP （50- 50000ng.mL-1）, ADM （20~ 20000ng-mL-1） with no significant difference （P 〉 0.05）, compared with single series concentration of 5--FU, DDP and ADM. Inducing expression of MDR1 gene were found for K562, but not for SPC--A--1 cells after exposure of 1 -100ng.m-1 nicotine. Conclusion：Nicotine is able to promote growth of SPC--A--1 cells, and significantly reduce chemosensitivity of SPC--A--1 cells to 1ng.mL--15--FU, but not DDP and ADM. And nicotine does not significantly induce expression of MDR1 gene for SPC--A--1 cells, either.
出处
《黑龙江医药科学》
2013年第1期9-11,共3页
Heilongjiang Medicine and Pharmacy
关键词
尼古丁
肺癌
化疗敏感性
体外实验
多药耐药基因
nicotine
lurig cancer
chemosensitivity
in vitro experiment
multidrug resistance gene
