摘要
通过模拟畜禽胃肠道环境,对保藏的9株木聚糖酶产生菌的粗酶液分别进行酸处理、胃蛋白酶处理以及胰蛋白酶处理,测定木聚糖酶的残余活力,结果发现,多数菌株所产木聚糖酶对酸和胃蛋白酶的稳定性较差,残余酶活力分别在50%以下和20%以下;而不同木聚糖酶对胰蛋白酶的稳定性差异较大,残余酶活力在30%～90%。在所有菌株中,黑曲霉3092和UV11所产木聚糖酶对酸和蛋白酶的稳定性都较好,适合在胃肠道环境中作为饲料添加剂使用。
The residue activities of the crude xylanases of nine mold strains were determined after the enzyme preparation were exposed to acid,pepsin and typsin,repectively,simulating the fowl gastrointestinal environment.The results show that there are lower stabilities to acid and pepsin,with less than 50% and 20% of residue xylanase activities,repectively.However,the stabilities of these xylanases to trypsinase are evidently different,with a range from 30% to 90% of residue xylanase activities.Amongst all the strains,Xylanses produced by Aspergillus niger 3092 and Aspergillus niger UV11 were more resistant to both acid and protease,suggesting good performance in the digestive tract as a feed supplement.
出处
《河南农业科学》
CSCD
北大核心
2012年第6期152-154,共3页
Journal of Henan Agricultural Sciences
基金
河南省科技厅重点攻关项目(0423021600)
