摘要
采用滤纸片法测定海洋真菌梅花状青霉(Penicillium herquei)FS83的发酵提取物对金黄色葡萄球菌(Staphylococcusaureus)、枯草芽孢杆菌(Bacillus subtilis)、铜绿假单胞菌(Pseudomonas aeruginosa)和大肠杆菌(Escherichia coli)的抑菌活性,采用生长速率法测定提取物对绿色木霉(Trichoderma viride)、黑曲霉(Aspergillus niger)、黄曲霉(Aspergillus flavus)、链格孢(Alterna-ria alternata)和胶孢炭疽菌(Colletotrichum gloeosporioides)的抗真菌活性,并采用连续稀释法测定提取物对所有细菌和真菌的最低抑制浓度(MIC值)。此外,还通过MTT法测试提取物对人肺癌细胞(NCI-H460)、人乳腺癌细胞(MCF-7)和人神经胶质瘤细胞(SF-268)的细胞毒活性。结果表明,菌丝体提取物对金黄色葡萄球菌和枯草芽孢杆菌有明显的抑制活性,抑菌圈分别为18.1 mm、17.3 mm,最低抑制浓度均为1.56 mg/mL,对所有供试真菌也有较好的抑制作用,抑菌率均达50%以上,最低抑制浓度为0.78-3.12mg/mL;发酵液提取物对枯草芽孢杆菌有明显的抑制作用,抑菌圈达16.4 mm,最低抑制浓度均为1.56 mg/mL,对所有供试真菌也有较好的抑制作用,最低抑制浓度为1.56-6.25 mg/mL。菌丝体提取物对3种肿瘤细胞株有较强的细胞毒活性,IC50值为55.9-63.5μg/mL,发酵液提取物对肿瘤细胞株MCF-7有较强的细胞毒活性,在200μg/mL浓度下的抑制率为84.3%。
The mycelial and broth extracts of a marine-derived strain of the fungus Penicillium herquei FS83 were tested for their antibacterial activities against Staphylococcus aureus, Bacillus subtilis, Pseudomonas aeruginosa and Escherichia coli by filter paper method, and investigated for their antifungal activities against Trichoderma viride, Aspergillus niger, Aspergillus flavus, Alternaria alternate and Colletotrichum gloeosporioides by growth rate method. Minimum inhibitory concentrations （ MICs ） of the culture extracts were determined by serial dilution method. Furthermore, the extracts were evaluated for their cytotoxic activities against NCI-H460, MCF-7, and SF-268 tumor cell lines by the MTT method. The results showed that the mycelial extract displayed significant inhibitory effect on S. aureus and B. subtilis with inhibition zone diameters of 18.1 and 17.3 mm respectively, and the same MIC of 1.56 mg/mL, and also exhibited satisfactorg antifungal activities against all the test fungi with inhibitory rates of over 50% and MICs in the range of 0.78-3.12 mg/mL. The broth extract showed a remarkable antibacterial effect on B. subtilis with an inhibition zone diameter of 16.4 mm and an MIC of 1.56 mg/mL, and also displayed antifungal activities on all the test fungi with MICs in the range of 1.56-6.25 mg/mL. The mycelial extract had a strong cytotoxic activity against the three tumor cell lines with IC50 values between 55.9-63.5 μg/mL, and the broth extract showed a strong cytotoxic activity against MCF-7 cell line with an inhibition rate of 84.3% at a concentration of 200μg/mL.
出处
《生物技术通报》
CAS
CSCD
北大核心
2013年第1期151-155,共5页
Biotechnology Bulletin
基金
广东省科技计划项目(2011B031200003
2010B030600010)
中国科学院知识创新工程重要方向项目(KSCX2-EW-G-12B)
关键词
海洋真菌
梅花状青霉
提取物
抑菌活性
细菌
真菌
细胞毒活性
Marine fungus Penicillium herquei Extract Antimicrobial activity Bacteria Fungi Cytotoxic activity
