摘要
目的通过18F-脱氧葡萄糖（FDG）细胞结合率的变化来早期评价5．Fu对人乳腺癌细胞MCF．7的疗效。方法不同浓度（0．125、0．25、0．5、1．0mg／mL）5-FU1．0mL分别作用于人乳腺癌细胞株MCF-724h、48h后，测定细胞摄取”F．FDG的变化，并与MTT法测定的存活细胞数目和细胞抑制率比较。结果5-FU作用24h和48h后，18F-FDG细胞摄取率均随药物浓度的增加而下降，两者的细胞摄取率与药物浓度均呈负相关（r=0．685，r=0．690，P〈0．01），并且48b比24h18F．FDG细胞摄取率低，两者MTT细胞抑制率与18F-FDG细胞结合抑制率均呈正相关（r=0．600，r=0．841，P〈0．01）。结论18F-FDG摄取变化可以早期评价5-Fu对MCF．7的疗效；随着5．Fu化疗时间的延长，人乳腺癌细胞株MCF-7细胞摄取率下降更明显。因此18F-FDGPET或PET／CT有望成为早期评价化疗对乳腺癌敏感性的标准之一。
Objective To evaluate the effect of 5-FU early by uptake of 18F-FDG in human breast cancer MCF-7 cells.Methods The proliferation inhibition effect by MTT and the uptake rates of 18F-FDG were detected after treatment with 5-FU for 24h and 48h.Results The uptake rates was negatively correlated with the dose of 5-FU at 24h and 48h after administration of 0.125,0.25,0.5 and 1.0mg/mL 5-FU（r=-0.685,r=-0.690,P 〈 0.01） and the uptake rates of ~SF-FDG with human breast carcinoma cell MCF-7 is lower at 48h after administration of 5-FU than at 24h.The binding inhibition efficiency was positively correlated with the proliferation inhibition（r=0.600,r=0.841,P 〈 0.01）.Conclusions The study indicated that early therapeutic effect of 5-FU for breast cancer cell MCF-7 could be assessed by 18F-FDG.Thus 18F-FDG PET or PET/CT could evaluate the early chemotherapeutic effect of breast cancer.
出处
《中国血液流变学杂志》
CAS
2012年第4期576-578,728,共4页
Chinese Journal of Hemorheology
