摘要
目的:优化从蛹虫草中提取、纯化虫草素的方法并鉴定其纯度。方法和结果:通过3种提取方法的比较,确定了以恒温水浴法作为虫草素规模化提取方法,最佳提取条件为45℃、80%乙醇;同时,确定了ML-7大孔树脂纯化虫草素的工艺,采用该工艺虫草素纯度可达到92%以上,并对纯化虫草素和标准品进行了比较鉴定。结论:该方法提取、纯化的虫草素纯度高,适于虫草素的规模化生产。
Objectlve：To optimize the technology of the extraction and purification of cordycepins from Cordyceps militaris and identify the purity of cordycepin. Method and Result ：the optimum conditions of the water- bath extraction method were the temperature of 45℃ and ethanol concentration of 80% compared with the methods of ultrasonic extraction and microwave extraction were studiod in the paper. Furthermore,purification technologic conditions of cordycepins with ML -7 macroperous resin were determined and the purity of cordycepins could reach 92% compared with the standard sample. Conclusion：this study supplied an effective method to obtain high -pu- rity cordycepin that it was feasible to produce cordyeepin by large- scale.
出处
《生物技术》
CAS
CSCD
北大核心
2012年第5期75-78,共4页
Biotechnology
基金
大连市科技计划项目("北冬虫夏草有效成分的提取及抗肿瘤作用"
2009E11SF130)资助
关键词
蛹虫草
虫草素
提取
大孔树脂
纯化
Cordyceps militaris
cordycepins
extraction
macroporous resin
purification
