摘要
选用21日龄杜长大仔猪60头,随机分成3组,每组20头。①哺乳组:不断奶继续哺乳;②断奶组;③试验组:断奶前1h腹腔注射P38MAPK通路抑制剂(SB203580)2mg/kg,之后隔日同一时间同剂量注射。仔猪21日龄断奶,试验期为7d。分别于21,22,24,28日龄屠宰仔猪取样待测。结果表明,在22和24日龄时,试验组绒毛高度分别高于断奶组12.06%和13.49%,差异显著(P<0.05);24和28日龄时,试验组隐窝深度分别低于断奶组11.21%和12.12%,差异显著(P<0.05);22,24,28日龄时,试验组绒毛高度隐窝深度之比显著高于断奶组(P<0.05)。试验组血浆D-乳酸含量和DAO活性在22,24,28日龄时均显著低于断奶组(P<0.05)。与断奶组相比,试验组显著降低了22日龄空肠、24日龄和28日龄回肠肠黏膜TNF-α的含量(P<0.05),而与哺乳组相比差异不显著(P>0.05)。试验结果提示:阻断P38MAPK通路可能通过降低肠黏膜TNF-α的产生,缓解断奶仔猪的肠道炎症,保护肠黏膜屏障;P38MAPK信号通路在断奶应激致小肠黏膜屏障受损过程中起调控作用。
Weaning has been shown to damage the intestinal barrier integrity,the mechanisms have not yet been elucidated. This experiment was conducted to investigate the role of P38 MAPK signaling pathway in the damage of mucosal barrier induced by weaning stress in piglets. Sixty piglets（Duroc× Landrace× Yorkshire） were randomly allocated to 3 groups. The three groups were： （ 1 ） unweaned group ; receiving sow milk, （2） weaned group ： weaning at d21, receiving basal diet, （ 3 ） experi- mental group ：weaning at d21, receiving basal diet with intraperitoneal injection SB203580 at 2 mg/kg and doing with same alternate day. Six piglets of each group were chosen to slaughter at d22,d24 and d28 to investigate the morphology,permeability and mucosa TNF-α of small intestine（two piglets of each group at d21）. The results showed that the experimental group had higher（P〈0.05） villus height and villus height/crypt depth,and plasma D-lactate and DAO activity were obviously lower （P^0.05） than the weaned group, intestine mucosa TNF-α content of experimental group was decreased compared with weaned group. In conclusion, inhibition of P38 MAPK signaling pathway has a protective effect on damage of intestinal mucosal barrier induced by early weaning through decreasing the content of intestine mucosa TNF-α. P38 MAPK signal pathway has a regulation function on the process of enterocyte tight junction damage induced by weaning stress.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2013年第2期302-306,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(31072039)
浙江省自然科学基金资助项目(Z3100071)
国家"948"计划资助项目(2011-G7-1)
