摘要
目的通过分析胃癌、胃癌前病变及胃良性病变组织中肥大细胞数目分布,了解肥大细胞与肿瘤形成及生存期的关系。方法回顾性分析59例胃癌、40例胃癌前病变、39例胃良性病变患者的临床病理及随访资料。采用免疫组化标记肥大细胞特异性类胰蛋白酶,分析肥大细胞在胃癌(高、中、低分化)组织中的数目及分布,并与胃癌前病变、胃良性病变组织中的表达进行比较,同时分析其与临床病理资料及生存期的关系。结果胃癌、胃癌前病变组织中肥大细胞数明显高于胃良性病变(P均<0.05),胃癌前病变组织中肥大细胞数明显高于胃癌(P<0.05)。将胃癌组和胃癌前病变组分别以肥大细胞数中位数(28、43)分组比较,胃癌、胃癌前病变中肥大细胞高水平组和低水平组生存期比较差异无统计学意义(P均>0.05)。肥大细胞数与幽门螺杆菌感染及Ki-67表达强弱有明显相关性(P均<0.05)。结论胃癌、胃癌前病变、胃良性病变组织中肥大细胞差异提示肥大细胞可能参与了早期肿瘤的形成,特别是幽门螺杆菌感染诱发的胃癌;肥大细胞与Ki-67的关系提示其促进肿瘤增殖与进展,但是与肿瘤分化程度无关;同时,肿瘤组织中肥大细胞与患者生存期无明显相关性。
Objective To investigate the correlation of mast cell with tumorigenesis and survival time through analyzing the mast cell counts in the tissues of gastric carcinoma, precancerous lesions of gastric carcinoma and benign gastric lesions. Methods The pathological and follow-up data of 59 patients with gastric carcinoma, 40 patients with precancerous lesions of gastric carcinoma and 39 patients with benign gastric lesions were analyzed retrospectively. The mast cell specific tryptase was labeled by immunohistochemistry. The counts and distribution of mast cells in the tissues of gastric carcinoma （high, middle and low differentiation） were analyzed. The expression in the tissues of gastric carcinoma was compared with those in the tissues of precancerous lesions of gastric carcinoma and benign gastric lesions, and the correlation of mast cell with pathological data and survival time was analyzed simultaneously. Results The mast cell counts were higher in the tissues of gastric carcinoma and precancerous lesions of gastric carcinoma than those of benign gastric lesions （P 〈 0.05 and P 〈 0.05）, and the mast cell counts in the tissues of precanerous lesions of gastric carcinoma were higher than those of gastric carcinoma （ P 〈 0.05 ）. According to the medians of mast cell counts （28,43）, the results between gastric carcinoma group and precancerous lesions of gastric carcinoma group were compared, and the survival times of high-level and low-level mast cell counts in gastric carcinoma and precancerous lesions of gastric carcinoma groups had no statistical significance （P 〉 0.05 and P 〉 0.05 ）. There were correlations of mast cell counts with Helicobacter pylori and Ki-67 expression （P 〈 0.05 and P 〈 0.05 ）. Conclusions The varied mast cell counts show that mast cells correlate the protumorigenesis, especially Helicobacter pylori induced gastric carcinoma. The correlation of mast cell and Ki-67 shows mast cell exert tumor proliferation and progression, but there is no correlation with
出处
《检验医学》
CAS
2013年第1期67-71,共5页
Laboratory Medicine
基金
国家自然基金面上项目(81072448)
上海市科委基础处重点项目(11JC1410300)
上海交通大学重大项目培育基金资助项目
关键词
肥大细胞
胃癌
癌前病变
幽门螺杆菌
生存期
Mast cell
Gastric carcinoma
Precancerous lesion of gastric carcinoma
Helicobacter pylori
Survival time
