摘要
目的:检验糖尿病候选基因在中国人群中与2型糖尿病的相关性。方法:以1 463名无血缘关系的中国天津地区人群为研究对象[其中对照组(非糖尿病)548名,病例组(2型糖尿病)915名]进行病例-对照研究。采用MALDI-TOF MS对17个基因的21个SNP位点进行基因分型,运用PLINK软件进行关联分析及基因型交互作用分析。结果:发现5个SNP位点与2型糖尿病显著相关(P<0.05),分别为CDKN2A/B(rs10811661,P=2.91×10-5)、CDKAL1(rs7756992,P=0.0028)、LPHN3(rs6856526,P=0.0031)、IGF2BP2(rs4402960,P=0.0006)和ABCG2(rs2231142,P=0.0315);并发现6对基因间的交互作用达到显著水平(P<0.05)。结论:LPHN3和ABCG2基因的多态性与2型糖尿病相关;并进一步验证了CDKN2A/B、CDKAL1、IGF2BP2基因的多态性在中国人群中与2型糖尿病的关联性。
Objective： To identify associations of diabetes candidate genes with type 2 diabetes in Chinese population. Methods： 1 463 unrelated Chinese from Tianjin in a ease-control study （non-diabetes controls, n=548; type 2 diabetes cases, n=915） were examined. 21 single nueleotide polymorphisms （SNPs） in 17 genes were genotyped by MALDI-TOF MS, PLINK software was used for association aml pairwise genotype interaction analyses. Results： Five genes were significantly associated with type 2 diabetes （P〈0.05）, including CDKN2A/B （rsl0811661, P=2.91 × 10-5）, CDKAL1 （rs7756992, P=0.0028）, LPHN3 （rs6856526, P=-0.0031）, IGF2BP2 （rs4402960, P=0.0006）, and A BCG2 （rs2231142, P=O.0315）; Genotype interactions （epistasis） among 6 pairs of SNPs were significantly associated with type 2 diabetes （P〈0.05）. Conclusion： In this study, the polymorphisms of LPHN3 and ABCG2 gene are associated with type 2 diabetes; previous associations among CDKN2A/B, CDKAL1, and IGF2BP2 gene polymorphisms and type 2 diabetes are replicated in Chinese population.
出处
《天津医科大学学报》
2013年第1期6-8,12,共4页
Journal of Tianjin Medical University
基金
国家自然科学基金资助项目(81070576)
天津市自然科学基金重点项目(12JCZDJC 24700)
关键词
2型糖尿病
单核苷酸多态性
关联分析
基因型交互作用
type 2 diabetes
single nucleotide polymorphism
association analysis
genotype interaction
