摘要
目的考察新风胶囊对干燥综合征模型大鼠肺功能的作用,及其对TGF-β1-ERK1信号通路的影响。方法将50只SD大鼠随机分为正常对照组、模型对照组和羟氯喹(HCQ)组、白芍总苷(TGP)组、新风胶囊(XFC)治疗组,每组10只,除正常对照组外,采用完全弗氏佐剂+同种鼠颌下腺抗原诱导方法,向每只大鼠两后足跖部注射与弗氏完全佐剂充分乳化后的颌下腺蛋白混合抗原0.2 mL诱发大鼠干燥综合征模型。用动物肺功能仪检测大鼠肺功能,检测各组大鼠饮水量及体质量的变化、采用免疫组化法ERK1、TGF-β1的表达,采用ELISA法检测血清细胞因子(IL-17、IL-4)的变化。结果与正常对照组(NC)比较,模型对照组(MC)大鼠体质量、血清IL-4明显降低,饮水量、颌下腺/肺指数、颌下腺病理评分、ERK1、TGF-β1积分及IL-17升高(P<0.01或P<0.05),肺功能参数降低(P<0.01或P<0.05);与MC组比较,XFC组体质量、肺功能参数50%肺活量的最大呼气流量(FEF50)、最大呼气中段流量(MMF)升高,IL-4表达升高,饮水量、颌下腺/肺指数、颌下腺病理评分、血清IL-17的表达、ERK1、TGF-β1积分降低(P<0.01或P<0.05)。与HCQ组相比,XFC组体质量、IL-17明显降低(P<0.01),FEF25、FEF75、MMF升高(P<0.01或P<0.05);与TGP组比较,XFC组肺指数、IL-17降低(P<0.01或P<0.05)。结论 SS大鼠存在肺功能下降,可能与TGF-β1-ERK1信号通路活化相关。中药XFC能够下调TGF-β1、抑制ERK1磷酸化、降低免疫炎症反应、改善肺功能。
Objective To explore the effect of XinFeng capsule（XFC） on pulmonary function of Sjgren syndrome（SS） rat and on the TGF-β1-ERK1 signal pathway.Methods A total of 50 SD rats were randomly divided into normal control group,model control group and hydroxychloroquine sulfate（HCQ） group,radix paeoniae alba（total glucosides of paeony capsules,TGP） group,XFC treatment group,with 10 in each group.Except for the normal control group,the other group rats were induced to establish SS models by injecting the complete Freund’s adjuvant and the same rat submandibular gland antigen（0.2 mL mixture） into bilateral posterior paw metatarsus.All the animals were examined with lung function detection instrument.Meanwhile,we recorded the changes in water consumption and body mass of the rats and observed the expressions of ERK1 and TGF-β1 using immunohistochemistry and serum cell factors（IL-17,IL-4） using ELISA.Results Compared with the normal control group,the model controls had reduced body mass,lower IL-4 level,increased water consumption,submandibular gland/lung index,submandibular gland pathological score,ERK1,TGF-β1 integral and IL-17 level（P0.01 or P0.05）,and decreased pulmonary function parameter（P0.01 or P0.05）.Compared with model control group,in XFC treatment group body mass,pulmonary function parameter FEF50,maximum midexpiratory flow rate（MMF）,and IL-4 expression level increased,while water consumption,submandibular gland/lung index,submandibular gland pathological score,serum IL-17 expression,ERK1,TGF-β1 integral decreased（P0.01 or P0.05）.XFC group rats were elevated in FEF25,FEF75,MMF（P0.01 or P0.05） and lowered in body mass and IL-17 level（P0.01） as compared with the HCQ group,and the pulmonary function parameters and IL-17 level all went down as compared with the TGP group（P0.01 or P0.05）.Conclusion Lung function of SS rats degrades,which may be related to TGF-β1-ERK1 signal pathway activation.XFC can down-regulate TGF-β1 level,inhibit ERK1 phosphorylation
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2013年第2期118-122,共5页
Chinese Journal of Cellular and Molecular Immunology
基金
国家中医药重点学科中医痹病学建设项目(国中医药发[2009]30号)
安徽省科技厅科研计划项目(09020304046)
