摘要
目的:研究BVT.2733对MCP-1表达的影响以及探讨其改善胰岛素抵抗的作用机制。方法:建立高脂饮食诱导的肥胖小鼠模型,随机分为三组:正常对照组、肥胖对照组、BVT.2733治疗组。分别给予安慰剂、BVT.2733灌胃14天,采用生化法检测血糖,放免法检测小鼠空腹胰岛素水平,Real-time PCR检测内脏脂肪中MCP-1的mRNA表达。观察各组胰岛素抵抗相关指标表达差异情况。结果:肥胖组小鼠体重明显增加,空腹血糖及胰岛素水平升高(P<0.05),形态学上发现小鼠脂肪细胞体积增大。与肥胖对照组相比较,治疗组小鼠脂肪细胞体积减小,空腹胰岛素水平下降明显(P<0.01),脂肪组织MCP-1 mRNA表达明显降低(P<0.05)。结论:BVT.2733能够降低MCP-1的表达水平,其可能通过抑制炎症来改善胰岛素抵抗。
Objective：To investigate the association between BVT.2733 and Monocyte Chemotactic Protein-1 in mice with diet-induced obesity.Methods：The mice were randomly divided into the normal group and high-fat group.After fourteen days,they were randomly divided into two groups：obese control group,BVT.2733 group.They were orally administered placebo and BVT.2733 for 14 days.The levels of serum insulin and plasma glucose were measured by Radioimmunity and Biochemistry technology.The adipocyte sizes were detected by HE satining.The expression of MCP-1 was analyzed by real-time quantitative PCR.Results：In HFD group,the weight,serum insulin and plasma glucose levels increased（P0.05）,and the adipocyte sizes became bigger.In BVT.2733 group,the serum insulin significantly decreased（P0.01）and the adipocyte sizes lessened,while the mRNA expression of MCP-1 down-regulated（P0.05）.Conclusion：BVT.2733 could down-regulate the expression of MCP-1,improve insulin resistance by inhibiting inflammation.
出处
《中国医药导刊》
2012年第12期2165-2166,共2页
Chinese Journal of Medicinal Guide
