摘要
目的探讨恐伤母鼠对仔鼠空间学习和记忆能力影响。方法采用旁观电击法制备恐伤母鼠动物模型,应用Morris水迷宫定位航行实验及空间探索实验测试1月龄仔鼠空间学习和记忆能力。结果定位航行实验中,对照组1～4 d平均潜伏期分别为(47.7±16.7)、(39.8±20.6)、(30.9±18.9)、(26.6±13.3)s,模型组分别为(89.5±27.2)、(70.7±40.0)、(57.4±38.3)、(55.5±35.9)s,模型组比对照组仔鼠平均潜伏期延长,差异均有统计学意义(P<0.05);与对照组比较,模型组仔鼠较多采用非有效策略,较少采用有效策略,差异有统计学意义(χ2=5.104,P=0.024);空间探索实验中,模型组比对照组仔鼠平均游泳速度变慢,游泳路程在20%边缘区平均百分比变大,在其他区域平均百分比减小(P<0.05)。结论恐伤母鼠可使仔鼠空间学习和记忆能力下降。
Objective To explore the effect of terror stress of pre-pregnant rats on the spatial learning and memory abilities of neonatal offspring. Methods The terror stress model of rats was established according to the method of watching other rats shocked by electricity, and the place navigation and probe trial testing of Morris water maze task were performed to assess the spatial learning and memory abilities of the offspring at the age of 1 month. Results In place navigation test for 4 days, the average latency time was 89.5 ± 27.2 s ,70. 7 ± 40. 0 s ,57.4 ± 38.3 s, and 55.5 ± 35.9 s in the model group, while it was 47.7 ± 16. 7 s,39. 8 ± 20. 6 s, 30. 9 ± 18.9 s, and 26. 6 ± 13.3 s in the control group, respectively. Compared with the control group, the mean escape latency was prolonged in the model group, with significant difference （ P 〈 0. 05 ）. Compared with those in the control group, the neonatal offsprings in the model group chose noneffective strategies more frequently, with significant difference （X^2 = 5. 104, P = 0. 024）. In probe trial testing, compared with the control group, the average speed of the model group was slower and the percent of swimming distance within the 20% rim quadrant was larger, but smaller in other quadrant（ P 〈 0. 05 ）. Conclusion Terror-stimulated adult female rats could induce decreased spatial learning and memory abilities in their offsprings.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第2期214-216,共3页
Chinese Journal of Public Health
基金
国家自然科学基金(81072734)
河南省科技创新人才项目(114100510011)
河南省教育厅自然科学基金(2010A360027)
校创新人才项目
河南中医学院苗圃工程项目
关键词
恐伤母鼠
仔鼠
学习记忆
MORRIS水迷宫实验
terror-stimulated adult female rat
neonatal offspring
learning and memory abilities
Morris water maze
