摘要
为筛选绵羊肺炎支原体(Mo)最适生长培养基,针对Mo 16S rRNA基因设计特异性引物FQs/FQa,建立可定量检测Mo 16S rRNA基因FQ-PCR方法,通过检测不同培养时间各Mo培养液中Mo Y98株核酸含量,比较Mo于不同培养基中培养特性,以筛选Mo最适生长培养基。结果,以Broth-ame与TSB1培养Mo 7,10和14 d时Mo核酸检测值较高,较Frey-ame、PPLO-ame更适合Mo生长。本研究为后续Mo生物学特性分析奠定了基础。
To select the best growth medium of Mycoplasma ovipneumoniae （ Mo）, the experiment was conducted to compare the culture characteristics of Mo in different media by detecting nucleic acid contents of Mo Y98 strain. A pair of primers FQs/Fqa was designed to am- plify 16S rRNA gene of Mo by fluorescent quantitative PCR. The results showed that Mo grew better in Broth-ame and TSB1 rather than in Frey-ame and PPLO-ame, as the nucleic acid detecting values of Mo at 7d, 10d and 14d were higher. This study provides a foundation for understanding the biological characterization of Mo.
出处
《畜牧与兽医》
北大核心
2013年第1期9-14,共6页
Animal Husbandry & Veterinary Medicine
基金
贵州省农业科技攻关项目(黔科合NY字[2011-3105]号)
贵州省自然科学技术基金(黔科合J字[2011]2332号)
贵州大学引进人才科研项目(贵大人基合字(2010)35号)
黔西南州种草养羊产业发展省州科技合作专项项目(黔西南科合[2012]5号)
贵州大学2012年研究生创新基金项目(校基金农科2012034)
