摘要
目的通过研究干扰素/维甲酸联合应用诱导细胞凋亡相关基因(GRIM-19)在小鼠植入前胚胎中的表达及其与胚胎质量之间的相关性,探讨GRIM-19在小鼠植入前胚胎发育中的作用。方法采用实时定量PCR,检测小鼠植入前胚胎(2-细胞期、4-细胞期、8-细胞期、桑葚胚和囊胚)中GRIM-19 mRNA水平(n=16);将小鼠8-细胞期胚胎按卵裂球大小、形态、透明带、胞质碎片分为优胚组和非优胚组,分别检测两组胚胎GRIM-19mRNA水平,并分析其相关性(n=13);制作假孕鼠(23只),并随机分为A组(12只)和B组(11只),采用移植器将优质和非优质8-细胞期胚胎移入假孕鼠的子宫内,观察两组雌鼠妊娠率及产仔率。结果 GRIM-19在小鼠植入前各期胚胎中均有表达,其mRNA水平从2-细胞期逐渐增高,至8-细胞期达高峰,随后呈下降趋势。优质8-细胞胚胎的GRIM-19 mRNA水平明显高于非优胚组(P<0.05)。两组8-细胞胚胎移植后,A组雌鼠妊娠率及产仔率显著高于B组雌鼠(P<0.05)。结论小鼠植入前胚胎中GRIM-19的表达量随发育时程的改变而变化,且与胚胎质量密切相关。
Objective To study the expression of gene associated with retinoid-interferon-induced mortality-19 （GRIM-19） and its correlation with the embryo quality, and to explore the effect of GRIM-19 in the development of preimplantation embryo of mice. Methods The expression of GRIM-19 in 2-cell, 4-cell, 8-cell, morula and blastocyst of mice preimplantation embryo was detected by using real-time PCR （n = 16 ）. Eight-cell embryos were divided into two groups： good quality embryos and bad quality embryos, according to their size, the pellucid zone and the debris of neoplasm. The different levels of embryos were detected （n = 13）. The expression of GRIM-19 in different groups were analyzed statistically. Results GRIM-19 expressed in every stage of preimplantation embryo of mice. The results of real- time PCR showed a gradually increase of GRIM-19 expression from 2-cell to 8-cell, and the expression then decreased progressively. In 8-cell embryo, mRNA expression of GRIM-19 in good quality embryos was significantly higher than that in bad quality embryos （P 〈 0. 05）. Conclusion The expression of GRIM-19 in mouse preimplantation embryos is changed with the progress in development. GRIM-19 may reflect the potentiality of embryo development.
出处
《解剖学报》
CAS
CSCD
北大核心
2013年第1期97-101,共5页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30901603)
山东省优秀中青年科学家科研奖励基金资助项目(BS2009SW031)
山东省人口和计划生育委员会科学技术研究资助项目(2009年第8号)
