摘要
目的探讨环境雌激素壬基酚(NP)暴露对雌性SD大鼠胸腺组织及胸腺淋巴细胞的损伤作用。方法将32只健康8～9周龄SPF级雌性SD大鼠按体重随机分为溶剂对照组(玉米油)和低(20 mg/kg)、中(80 mg/kg)、高(200 mg/kg)剂量NP组,每组8只,采用灌胃方式染毒,灌胃容量5 ml/kg,隔天染毒1次,连续60 d,观察胸腺组织病理学改变;采用流式细胞仪检测胸腺淋巴细胞内活性氧(ROS)、游离钙离子([Ca2+]i)浓度、线粒体膜电位(MMP)水平变化。结果溶剂对照组大鼠胸腺重量为(0.633±0.092)g,高剂量NP组为(0.514±0.084)g,差异有统计学意义(P<0.05),胸腺重量和染毒剂量呈负相关(r=-0.317,P<0.05);溶剂对照组与高剂量NP组大鼠胸腺淋巴细胞内ROS水平分别为(4.59±0.25)和(5.57±0.36),差异有统计学意义(P<0.05),胸腺淋巴细胞内ROS水平与NP浓度呈正相关(r=0.486,P<0.05);病理学检查可见,中、高剂量NP组胸腺皮质变薄。结论 NP对雌性大鼠胸腺及胸腺淋巴细胞具有一定毒性作用,并可引起免疫功能损伤。
Objective To study adverse effects of nonylphenol（NP） on thymus tissue and thymocytes of female Sprague-Dawley（SD） rats,and to investigate the effects of NP on immune function of female SD rats.Methods Thirty-two female SD rats were randomly divided into four groups（8 in each group） and treated with NP through gavage at the doses of 0,20,80,and 200 mg/kg once two days for 60 consecutive days.At the end of the experiment,the pathological examination of the thymus was done and the level of reactive oxygen species（ROS）,intracellular free calcium concentration（i）,and mitochondrial membrane potential（MMP） in thymus lymphocytes were detected with flow cytometry.Results Compared with the control,the rats＇ thymus weight significantly decreased in 200 mg/kg NP treated group,with a singnificant difference（P0.05）.The thymus weight correlated negatively with exposure concentration（r=-0.317,P0.05）.The levels of ROS in thymus lymphocytes were increased dramatically in 200 mg/kg NP treated group in a dose-dependent manner（r=0.488,P0.05）,with a significant difference（P0.05）.Histopathological changes were found in the thymus of the experimental groups with the thined cortex.Conclusion Exposure to nonylphenol by oral route may induce adverse effects on the thymus and thymus lymphocyte in rats and affect the immune function of female rats.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2013年第1期52-55,共4页
Chinese Journal of Public Health
基金
教育部留学回国人员基金(K5126504)
