摘要
单唾液酸四己糖神经节苷脂(GM1)是生物膜重要的组分之一,在神经系统病变治疗中起到重要的作用。本文通过分析GM1/二棕榈酰基磷脂酰胆碱(DPPC)混合脂单层膜等温线数据研究了脂膜分子间的相互作用机理,并利用原子力显微镜(AFM)对其形态进行了观测。实验结果表明:DPPC在混合脂中的比例含量XDPPC=0.2时,GM1与DPPC分子间的相互作用力为吸引力,膜较稳定;其它比例混合时分子间的相互作用力为排斥力,且在XDPPC=0.6时,分子间相互排斥力最大,单层膜出现了明显的富含DPPC的微区和GM1网状共存的结构。AFM形态观测与理论分析结果相一致。该研究为了解GM1在不同的膜脂系统中形态和功能的变化提供了重要的实验依据和理论支持。
Monosialotetrahexosylganglioside（GM1） is one of the most important components in the biological membrane and plays an important role in the nervous lesion treatment.This paper investigated the mechanism of the interaction between GM1 and 1,2-dipalmitoyl-sn-glycero-3-phosphocholine（DPPC） by analyzing the mixed lipids isotherms data.The morphology of monolayer was measured using atomic force microscopy（AFM）.The results show that the molecular interaction between GM1 and DPPC is attractive and the lipid monolayer is more stable when the proportion of DPPC in the mixed lipids is XDPPC=0.2.At the selected other ratios of DPPC,the molecular interaction is repulsive and the maximum locates at XDPPC=0.6.The coexistence structure of DPPC-enriched domains and GM1-enriched net can be observed from the AFM image.AFM observation results are consistent with the theoretical analysis.This research provides an important experimental basis and theoretical support for understanding the change of morphology and function of GM1 in different membrane systems.
出处
《电子显微学报》
CAS
CSCD
2012年第6期529-533,共5页
Journal of Chinese Electron Microscopy Society
基金
陕西省自然科学基础研究计划资助项目(No.2012JQ1002)
中央高校基本科研业务费专项资助项目(No.GK201102029)
