摘要
采用室内模拟试验的方法,以甲醇、稻壳和木屑3种材料作为有机碳源,通过在生物膜载体(秸秆炭化物)中添加有机碳源来提高对富营养化水体的净化效果。结果表明,在秸秆炭化物中分别添加了甲醇、稻壳和木屑处理(ZT-JC、ZT-DK、ZT-MX)对富营养化水体中总氮的移除率比只添加生物膜载体处理(ZT)分别提高了29.8%、14.3%、17.1%。另外,添加碳源的3个处理的水体中硝化细菌数量和反硝化细菌数量均高于ZT处理。在净化处理50 d后,ZT-JC处理水体中总有机碳(TOC)含量比对照(不添加载体材料和有机碳源处理)高了26.5%,表明液体碳源并不适合于富营养化水体的净化。PCR-DGGE检测结果显示,添加有机碳源处理的细菌多样性Shannon-Wiener指数和丰度均高于对照,其中添加稻壳处理的反硝化细菌数量多样性Shannon-Wiener指数明显高于添加木屑处理,表明稻壳比木屑更适合应用于富营养化水体的治理。
Three carbon sources （ methanol, rice hull, sawdust） were added into the biofilm carrier to improve nitro- gen removal from the eutrophic waters by indoor simulation experiments. The results showed that the removal rate of total nitrogen by methanol （ZT-JC）, rice hull （ZT-DK） and rice hull （ZT-MX）were 29.8%, 14.3%, 17.1% higher than that of control （ZT, no additional carbon sources）, respectively. The numbers of nitrifying bacteria and denitrifying bacteria in the treatments with exogenous carbon sources were higher than that in ZT treatment. After fifty days of purification, total or- ganic carbon content in ZT-JC treatment was 26.5 % higher than that in control （ no additional carbon sources and biofilm carrier） , indicating that liquid carbon source was inappropriate for the purification of the eutrophic water. PCR-DGGE pro- file revealed that the diversity index of Shannon-Wiener and richness in the treatments of additional carbon sources were in-creased, compared to that of control. The diversity index of Shannon-Wiener for the denitrifying bacteria in ZT-DK treatment was higher than that in ZT-MX treatment, indi- cating that the rice hull outperformed sawdust for the re- moval of nitrogen from eutrophic waters.
出处
《江苏农业学报》
CSCD
北大核心
2012年第6期1312-1317,共6页
Jiangsu Journal of Agricultural Sciences
基金
水体污染控制与治理科技重大专项(2011ZX07103-002)
关键词
碳源
富营养化水体
生物膜
细菌群落结构
carbon resource
eutrophic water
biofilm
bacterial community structure
