摘要
目的 探讨 St.Thomas' 心脏停搏液添加外源性磷酸肌酸 ( CP)对未成熟心肌的保护效果 ,并观察其抗心律失常作用 .方法 采用幼鼠 ( 18～ 2 1d)离体工作心模型 ,14℃低温缺血 12 0 min,缺血前主动脉根部灌注 4℃ St.Thomas' 停搏液 (对照组 )或 St.Thomas' 停搏液加 CP( 10 mmol.L- 1 ) ,观察缺血再灌注后心功能 .心肌 ATP含量的恢复百分比 ,心肌 CPK漏出量及再灌开始至窦性心律恢复的时间 .结果 1CP组主动脉流量恢复比明显增加 [( 86 .8± 4.2 ) %vs ( 70 .2± 3.3) % ,P<0 .0 1].2 ATP恢复百分比显著升高[( 90 .3± 6 .8) % vs( 72 .6± 5 .3) % ,P<0 .0 1].3心肌 CPK漏出量明显减少 ( P<0 .0 5 ) .4从再灌注开始至窦性心律恢复的时间显著缩短 [( 3.3± 1.5 ) min vs( 8.2± 2 .0 ) min,P<0 .0 5 ].结论 St.Thomas' 心脏停搏液中加外源性磷酸肌酸不仅可显著改善幼鼠低温缺血后心功能的恢复 ,促进高能磷酸盐的重建及减少心肌 CPK的漏出 ,而且具有较好的抗心律失常作用 。
AIM To determine whether creatine phosphate (CP) improves the protective effects of the St. Thomas' Hospital cardioplegia on immature myocardium and to evaluate the antiarrhythmic effects of CP. METHODS In an isolated working heart model, hearts from immature rats (18~21 days old) were subjected to 2 hours of 14℃ hypothermic ischemia with a 3 min preischemic infusion of either standard ST. Thomas'Ⅱ cardioplegic solution (control group) or the cardioplegia plus CP (10 mmol·L -1 ) (CP group). RESULTS The postischemic recovery of aortic flow was significantly better in the CP group than that in the CPfree control group (P<0.01). The percent recovery of myocardial ATP content was also higher in the CP group than that in the control group (P<0.01). The myocardial creatine phosphate kinase (CPK) leakage was significantly lower in the CP group than that in the control group (P<0.05). CP reduced reperfusion arrhythmias, significantly decreasing the time between cross clamp removal and return of regular rhythm. CONCLUSION Addition of CP to ST. Thomas'Ⅱ cardioplegia can significantly improve the functional recovery of immature myocardium following long period ischemia and attenuate postischemic myocardial arrhythmias.
出处
《第四军医大学学报》
2000年第5期523-526,共4页
Journal of the Fourth Military Medical University
基金
Partly supported by the grant of National NaturalScience Foundation (39500144)
关键词
磷酸肌酸
未成熟心肌
保护作用
心肌停搏液
creatine phosphate
immature myocardium
cardioplegia CLC number: R654.1 Document code: A
