摘要
目的探讨紧密连接蛋白Claudin-1、3、4、7在子宫颈正常组织、子宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)(包括CIN1、2、3)及浸润性鳞状细胞癌组织中的表达变化及意义。方法应用免疫组化SP法检测9例正常子宫颈组织、12例CIN1、15例CIN2、28例CIN3和22例浸润性鳞状细胞癌组织中Claudin-1、3、4、7的表达。结果从正常子宫颈组织→CIN1/2→CIN3→浸润性鳞状细胞癌,Claudin-1、3、4、7的表达逐渐增加。CIN3及浸润性鳞状细胞癌组织与正常组织及CIN1/2比较,差异有统计学意义(P<0.05)。CIN3与浸润性鳞状细胞癌比较,差异无统计学意义(P>0.05)。Claudin-1与Claudin-7、Claudin-3与Claudin-4的表达强度随疾病的进展呈明显正相关。结论 Claudin-1、3、4、7可能与子宫颈鳞状上皮的恶性转化相关,联合检测Claudin-1、3、4、7的表达可作为CIN和子宫颈鳞状细胞癌早期诊断的辅助指标。
Purpose To investigate the expression and significance of Claudin-1, 3, 4, 7 in normal cervical tissue, cervical intraepi- thelial neoplasia （CIN）, and invasive squamous cell carcinoma （ISCC）. Methods Expressions of Claudin-1,3, 4, 7 were detected by immunohistoehemistry in 9 cases of normal cervical tissue, 12 cases of CIN1 and 15 eases of CIN2, 28 cases of CIN3, 22 cases of ISCC. Results From the progress of normal cervical tissue→CIN1/2→CIN3→ISCC, the expression of Claudin-1, 3, 4, 7 increased gradually. Significantly increased expression of Claudin-1, 3, 4, 7 were detected in the CIN3 and ISCC compared with the normal tis- sue and CIN1/2 （P 〈0. 05）. There were no significant differences of Claudin-1, 3, 4, 7 expression between CIN3 and ISCC （P 〉 0. 05 ）. With the progression of cervical neoplasia, significant positive correlations were found between the expression of Claudin-1 and Claudin-7, Claudin-3 and Claudin-4. Conclusions The expression of Claudin-1, 3, 4, 7 may have an association with malignant transformation of cervical squamous epithelium. They maybe used as assistant index for early diagnosis of CIN and cervical carcinoma.
出处
《临床与实验病理学杂志》
CAS
CSCD
北大核心
2013年第1期49-52,57,共5页
Chinese Journal of Clinical and Experimental Pathology
