摘要
利用反相高效液相色谱法研究了武夷橙中7种有机酸的分离测定条件。采用C18为分析柱,以2.5%磷酸二氢铵缓冲溶液(PH=2.5)为流动相,流速为1mL/min,紫外检测波长在210nm处可以很好的分离测定有机酸。结果表明峰面积与相应的质量浓度有良好的线性关系,其r大于等于0.9990,平均回收率为98%～110%,该方法具有测定结果准确可靠,重现好等特点。
The condition of separation and detection of seven organic acids in Wuyi Orange by reversed-phase high performance liquid chromatography were studied .The organic acids can be separated and determined better in the conditions of 2.5% ammonium dihydrogen phosphate buffer solution（pH=2.5） as mobile phase, at a flow rate of 1mL/min and UV detection 210nm on a C18 column. The results showed that the area and the content were in a well linear correlation（r≧0.9990）, the average recovery was 98%～110%. This method also had the simple, well accuracy.
出处
《武夷学院学报》
2012年第5期35-37,共3页
Journal of Wuyi University
关键词
反相高效液相色谱法
武夷橙
有机酸
the reversed-phase high performance liquid chromatography
Wuyi Orange
organic acid
