摘要
目的探讨重组人促红细胞生成素（recombinant human erythropoietin，rhEPO）对大鼠急性。肾缺血再灌注损伤（ischemia—reperfusioninjury，IR／I）的保护作用及机制。方法21只健康雄性S—D大鼠随机分为假手术组（sham组）、IR／I组和rhEPO预处理组。双侧肾蒂夹闭45min后再灌注，建立大鼠IR／I模型。术后24h收集血液和肾脏标本，观察肾功能、肾脏病理和大鼠死亡情况；ELISA法检测血清血管内皮生长因子（VEGF）的水平；免疫组织化学法观察肾脏血红素加氧酶-1（HO-1）的表达。结果再灌注24h后，rhEPO预处理组Scr和BUN水平明显低于IR／I组（P〈0．01）；IR／I组出现肾小管上皮细胞广泛性坏死，rhEPO预处理组肾脏病理损伤程度较IR／I组明显减轻（P〈0．01）；IR／I组大鼠死亡率为28．6％（2／7），rhE—PO预处理组死亡率为0（0／7），Sham组死亡率为0（0／7）。rhEPO预处理组血清VEGF水平明显升高、肾脏HO一1蛋白表达明显增加，均高于IR／I组及sham组（P〈O．01）。结论rhEPO对急性肾脏IR／I有较好的保护作用，该作用可能是通过抗氧化、促进肾小管上皮细胞再生等的协同机制来实现。
Objectives To investigate protective effect of recombinant human erythmpoietin （rhEPO） on acute renal ischemia - reperfusion injury（IR/I） in rats. Methods Twenty one male S - D rats were randomly di- vided into 3 groups： Sham, IR/I, rhEPO（injection via intraperitoneal 2 h before surgery at the dosages of 100013/ kg）. Rat model of renal IR/I was established with clamping both pedicles for 45 min followed by reperfusion. Blood sample and kidneys were collected at indicated times. Serum creatinine levels, urea nitrogen, histological change and mortality were observed throughout the study. ELISA was used to measure the levels of serum vascular endothelial growth factor（ VEGF）. The expression of heme oxygenase - 1 （ HO - 1 ） in kidney observed by Results Extensive proximal tubular necrosis, functional impairment and higher mortality were found reperfusion after 24 hours in IR/I group（ P 〈0.01 ）. Elevated serum VEGF levels and increased expression of HO - 1 protein in kidney were significantly higher than IR/I group and sham group（ P 〈 0. 01 ）. Conclusions rhEPO can attenu- ate renal IR/I. The protection mechanisms may be through the anti - oxidation and to promote renal tubular epithelial cell regeneration.
出处
《国际泌尿系统杂志》
2013年第1期57-60,共4页
International Journal of Urology and Nephrology
关键词
再灌注损伤
肾
大鼠
红细胞生成素
重组
Reperfusion Injury
Kidney
failure
Rats
Erythropoietion, Recombinant
