摘要
本文采用葡聚糖包裹的活性炭石(DCC)法、羟基磷灰石(HAP)法及等电聚焦电泳(IEF)法,在各种条件下检测了1.25(OH)_2D_3(DHCC)受体的含量。这3种方法得到的表现解离常数(Kd)分别等于4.2×10^(-9)mol/L、2.3×10^(-9)mol/L和6.4×10^(-9)mol/L;该受体蛋白分别在10nmol/L、8nmol/L和30nmol/L浓度的〔~3H〕DHCC处被饱和。该受体对D_3类似物的结合能力依次为1,25(OH)_2D_3>>25(OH)D_3>D_3≈E_2。我们所测佝偻病鸡3种组织中该受体含量依次为肠>输卵管壳腺>>肝。用DCC法,在4℃孵育2～5h,结合最稳定。用KTED缓冲液测得的胞液DHCC受体含量比用TED缓冲液高(P<0.05)。
Three methods for the detection of DHCC receptor in vearious tissues were investigated. They were DCC assay , HAP assay and IEF assay. Values of Kd and R0 detected by DCC assay . HAP assay and IEF assay were 4. 2×10-9 and 6. 4×10-9 Analog competition for the receptor binding revealed the following order of potency : DHCC≥HCC≥CC≈E2. The contents of DHCC receptors in various tissues were ranked as follows: intestine >shell gland≥tiver. The time and temperature course of association fo DHCC with the cytoplasmic binder showed maximal steady state values between 2 and 5 h at 4 ℃. The content of DHCC receptor was significantly higher by KTED buffer assay than that of TED buffer assay.
出处
《白求恩医科大学学报》
CSCD
1991年第6期539-541,共3页
Journal of Norman Bethune University of Medical Science
