摘要
采用根癌农杆菌介导法,将ACC合成酶反义基因导入蝴蝶兰原球茎中,比较不同PPT筛选压力、Mer浓度和侵染时间等对蝴蝶兰转化的影响,优化蝴蝶兰的遗传转化体系.结果表明,在OD600值为0.2～0.3的农杆菌菌液中侵染120 min后,放入含有20 mg.L-1Mer和2.0 mg.L-1PPT的筛选培养基中的原球茎,遗传转化效率最高.
ACC synzyme gene was transformed into the PLBs of Phalaenopsis by using the agrobacteri- um mediated technique, screening PPT＇ s resistant pressure, and the meropenem concentration and im- mersed time on the transformation efficiency of Phalaenopsis PLBs were compared. The screening effi- cient genetic transformation system was improved. The results showed that after an Agrobacterium tume- faciens suspension （OD600 = 0.2 -0.3） was infected for 120min, the successfully infected PLBs using the screening medium which contained 20 mg. L-1 meropenem and 2.0 mg . L-1 PPT were deter- mined.
出处
《河南农业大学学报》
CAS
CSCD
北大核心
2012年第6期642-645,650,共5页
Journal of Henan Agricultural University
基金
河南省科技攻关项目(092102110128)
关键词
蝴蝶兰
ACC合成酶基因
遗传转化
Phalaenopsis
ACC synzyme gene
Agrobacterim mediated genetic transformation
