摘要
目的:研究采用微碱法进一步提取山药多糖复合物的最佳工艺条件。方法:以多糖提取率为指标,采用单因素试验和四因素三水平正交试验,研究提取时间(h),提取温度(℃),料液比(g/mL)和提取pH值对多糖复合物提取率的影响。结果:微碱法提取山药多糖复合物的最优工艺条件为:提取温度为50℃,提取时间为1.5 h,提取料液比(g/mL)为1∶15,pH值为10,此时山药多糖复合物最高提取率为5.739%。结论:优选的提取条件稳定可行。
Objective ：To optimize the extraction conditions of polysaccharide compound from yam under mild alkaline condition. Methods：Single factor test and orthogonal design test of L9（34）were adopted, and extraction rate of polysaeeharide was taken as index.Effects of extraction time,extraction temperature, solid-liquid ratio and pH value on extraction rate of polysaccharide were observed. Results ：The optimal conditions for yam by orthogonal test were achieved as follows： extracting for 90 min with extraction temperature at 50 ~C, solid-liquid ratio was at 1：15（g/mL）, pH value was at 10.Under above conditions,the extraction rate of yam polysaccharide was 5.739%. Conclusion：The optimized extraction conditions were stable and feasible.
出处
《山西中医学院学报》
2012年第5期25-28,共4页
Journal of Shanxi College of Traditional Chinese Medicine
关键词
山药
多糖复合物
正交试验设计
yam
polysaccharide compound
orthogonal design
