摘要
为了探讨桑叶提取物的抑菌活性及其物质基础,采用微量稀释法对桑叶70%乙醇提取物的4个萃取部位进行抑菌活性测试并利用气相色谱-质谱联用技术对抑菌活性强的萃取部位进行了成分分析,结果表明桑叶70%乙醇提取物4个萃取部位中石油醚部位抑菌活性最强,其次为乙酸乙酯部位,正丁醇部位和水部位。气相色谱-质谱检测分析石油醚萃取部位的主要成分及其质量分数为植物醇(24.87%)、环二十八烷(18.00%)、棕榈酸(10.20%)、α-亚麻酸(9.06%)、亚麻酸乙酯(7.92%)、硬脂酸(7.14%),另外还含有少量α-生育酚和β-谷甾醇等活性成分。
In order to explore the antimicrobial activity and material basis of mulberry leaves extract,the antibacterial activities of 4 fractions（petroleum ether,ethyl acetate,n-butanol and water）from muberry leaves crude extract were tested using micro-plate dilution assay.And the fraction of the strongest antibacterial activity was analyzed by gas chromatography-mass spectrometry.The results indicated that petroleum ether fraction had the strongest antibacterial activity,followed by ethyl acetate fraction,n-butanol fraction and water fraction.Then,the petroleum ether fraction was analyzed by gas chromatography-mass spectrometry.The result showed that this fraction was rich of phytol（24.87%）,cyclo octacosane（18.00%）,hexadecanoic acid（10.20%）,α-linolenic acid（9.06%）,ethyl linolenate（7.92%）,and octadecanoic acid（7.14%）.In addition,it also contained a small amount of α-tocopherol,β-sitosterol and other components.
出处
《食品工业科技》
CAS
CSCD
北大核心
2013年第1期117-120,共4页
Science and Technology of Food Industry
基金
国家现代农业产业技术体系建设专项资金(CARS-22)
广东省自然科学基金团队项目(粤科基字[2009]2号)
广东省社发重点项目(2011A030100003)
广东省科技成果转化启动项目(2010B060600005)
广州市科技计划重点项目(2011Y2-00009)
关键词
桑叶
抑菌
活性成分
muberry leaves
antibacterial
active component
