摘要
目的探讨缺血预处理对神经细胞凋亡的影响。方法采用四动脉阻断法大鼠全脑缺血模型，２１只Ｗｉｓｔａｒ大鼠，腹腔注射戊巴比妥钠麻醉。大鼠分四组：Ａ组，假手术对照组（ｎ＝５），未予脑缺血处理； Ｂ组，预处理对照组（ ｎ＝ ５），予 ３ｍｉｎ全脑缺血后未再次缺血； Ｃ组，预处理缺血组（ ｎ＝５），予３ｍｉｎ全脑缺血，再灌２４ｈ后，再次全脑缺血１０ｍｉｎ；Ｄ组，脑缺血组，予全脑缺血１０ｍｉｎ。各组动物均在再灌７２ｈ后断头取脑。采用流式细胞计数仪检测大鼠前脑皮质凋亡细胞的百分数。结果Ａ组的凋亡细胞百分数是（０．２６８０ ± ０．２０３５）％， Ｂ组（０．２７８０±０． １７８０）％， Ｃ组（０．３５００±０．０７４４）％， Ｄ组（０．６２８０±０．２１８１）％，Ａ、Ｂ及Ｃ组间无显著性差异（Ｐ＜０．０５），Ｄ组凋亡细胞百分数显著高于Ａ、Ｂ及 Ｃ组（ Ｐ＜ ０． ０５）。结论脑缺血预处理可抑制缺血诱导的神经细胞凋亡。
Objective To determine influence of the cerebral ischemia preconditioning on the neural apoptosis. Methods The whole cerebral ischemia was induced with four-blood vessel occlusion. Twentyone Wistar rats were randomly divided into four groups, sham--operation control group (group A, n = 5 ): without cerebral ischemia; cerebral ischemic preconditioning control group (group B, n = 5 ): 3 min cerebral ischemia only; cerebral ischemic preconditioning group (group C, n = 5): 3 min cerebral ischemia and 24 h reperfusion followed by cerebral ischemia 10 min; and cerebral ischemia group(group D, n = 6): cerebral ischemia 10 min. The apoptotic neural cells were detected by flow cytometry in the rat neocortex. Results The apoptotic cells percentages of group A, B, C and D were (0. 2680 ±0. 2035 ) %, (0. 2780 ±0. 1780) %, (0. 3500 ±0 .0744) % and (0. 6280 ±0 .2181 ) %, respectively. Without significant differeces between group A, B and C (P > 0 .05 ), but the apoptotic cells percentages were markedly in group A, B and C lower than in group D (P < 0.05 ) .Conclusions Cerebral ischemic preconditioning can suppress the apoptosis of neural cells following ischemia.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2000年第7期423-425,共3页
Chinese Journal of Anesthesiology
关键词
脑
缺血预处理
脱噬作用
Brain
Ischemic preconditioning
Apoptosis
