摘要
对形态结构基本一致的 3株苏云金杆菌噬菌体的核酸性质及其限制性内切酶的酶切特性进行了比较研究。甲酰胺 -细胞色素 C法进行分子展层 ,发现其核酸均呈线状 ;核酸的热变性试验和 S1 酶的电泳结果表明 ,所有核酸均为双链 ;Hind 和 Bam H 酶对 3株噬菌体核酸均不敏感 ;而它们均能被 Eco R 切割成 8个片段 ,被 Hpa 切割成 7个片段。经电泳法测定 ,其分子量均为 48.0
The properties of nucleic acids of three Bacillus thuringiensis phages with similar morphology from CGVCC and their digested characteristics by restriction endonuclease were compared Nucleic acid of all of 3 phages were linear by molecular spreading after treatment of formamide cytochrome C, and were double strand by thermal denaturetion and electrophoresis of enzyme S l DNAs of all 3 phages were not sensitive to restriction endonuclease HindⅢ and BamHⅠ,but were digested by EcoRⅠ and HpaⅡ into 8 and 7 fragments respectively The molecular weights of the phages′ DNAs were about 48 08kb
出处
《中国生物防治》
CSCD
2000年第2期69-73,共5页
Chinese Journal of Biological Control
关键词
苏云金杆菌
噬菌体
分子展层
限制性内切酶
核酸
Bacillus thuringiensis
bacteriophages
molecular spreading
restriction endonuclease pattern
