摘要
分析一对细胞或组织在不同状态下基因表达的差异,已成为分子生物学研究领域的热点之一.近年来,用于识别差异表达基因的方法已发展起来多种.DDRTPCR是近年来较为广泛应用的一种技术.理论上,DDRTPCR技术比较简单,但实施起来却存在着假阳性率高,凝胶中单条cDNA带成分不均一,所获cDNA仅代表着mRNA3′UT区(约300bp)以及一些低拷贝数mRNA不能有效被呈现等问题.对DDRTPCR技术的改良也主要集中在解决这些问题方面.
To identify and analyze differentially expressed genes between one pair of cells or tissues in different conditions is of great importance in molecular biology study. In recent years, a variety of approaches have been used to identify differentially expressed genes. DDRT PCR is one of the most widely used approaches. In theory, DDRT PCR technique is simple. But in practice, some limitations such as high false positive rate, inhomogeneity of cDNA bands, short cDNA fragments and underrepresentation of low abundance mRNAs exist. Most modifications to DDRT PCR technique mainly focus on above aspects.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2000年第1期28-32,共5页
Progress In Biochemistry and Biophysics
