摘要
目的：研究甘油二酯激酶ζ（DGKζ）在大鼠实验性自身免疫性脑脊髓炎（EAE）病理过程中的表达变化。方法：建立大鼠EAE模型，应用免疫蛋白印迹测定DGKζ表达量的变化，免疫组织化学和免疫荧光双标技术检测DGK℃在EAE不同病理阶段的脑干和脊髓中的细胞定位和分布的变化。结果：免疫蛋白印迹结果显示，脑干和脊髓中DGKζ在神经功能评分较低组（1～2分组）表达量均低于正常组，在神经功能评分较高组（3分和4分组）又显著升高，4分组与3分组相比无差异；免疫组织化学和免疫荧光双标结果显示，在正常组大鼠脑干和脊髓DGKζ均主要在神经元的细胞核阳性表达。在神经功能评分较低组，脑干和脊髓中DGKζ在神经元的表达减少，但在神经功能评分较高组DGKζ在神经元的表达又增多，并且在大量活化的小胶质细胞呈阳性表达。结论：DGKζ在EAE大鼠神经元和活化的小胶质细胞内表达变化提示DGKζ通过参与神经元和小胶质细胞的功能调节了该病理过程。
Objective： To investigate the expression changes of diacylglycerol kinase ζ （DGKζ） in the process of experimental autoimmune encephalomyelitis （EAE） in rats. Methods： The EAE rat model was performed. The expression, cellular locali- zation and distribution of DGKζ in EAE rat brainstem and spinal cord was detected by Western blotting, immunohistochemi- cal staining and immunofluorescent double-labeling staining. Results： In both brainstem and spinal cord, the expression of DGKζ in the clinic nerve function groups of a low score （score 1-2） was significantly lower than that in the normal group, and then increased significantly in the clinic nerve function groups of a high score （score 3 and 4 groups）. While there was no significant difference between score 3 and score 4 group; Immunohistochemical and immunofluorescent double-labeling stai- ning showed that DGKζ was expressed in the nucleus of the neuron in the normal rat brainstem and spinal cord. However, more and more neurons were DGKζ-negtive in the groups with a low score of clinic nerve functioru In the groups with a high score of clinic nerve function, DGKζ was expressed in the neuron and many actived microglias. Conclusion： The expression of DGKζ in the neuron and activated microglia was changed in rats with EAE, suggesting that DGKζ regulates the pathologi- cal process through being involved in the function of the neuron and microglia.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2012年第6期773-776,843,共5页
Chinese Journal of Anatomy
基金
山西省自然科学基金（2008011079-2）
山西医科大学科技创新基金（oi200719）
