摘要
目的:本研究旨在探讨血浆EB病毒DNA(Epstein-Barr virus DNA EBV DNA)拷贝数在鼻咽癌转移患者化疗疗效监测方面的临床意义。方法:选取2006年12月至2007年6月在中山大学肿瘤防治中心确诊的16例鼻咽癌转移患者,均接受6个疗程化疗。化疗方案以PF为主,辅以TP方案。每2个疗程化疗后外周静脉采血并采用影像学检查评价疗效。血浆EBV DNA检测采用荧光定量PCR法。结果:化疗后,EBV DNA下降者,疗效评价与EBV DNA变化基本一致,一致率达80%以上;升高者,疗效评价与EBV DNA变化高度一致,一致率达100%。而EBV DNA始终为0者,或在治疗过程中降为0者,疗效评价与EBV DNA变化不一致。根据疗效分为PR、SD、PD三组,将化疗前后EBV DNA拷贝数的对数值进行分析,三组分别为-4.63±2.5、-2.25±2.3、3.23±0,三组比较有显著性差异(P=0.025)。结论:血浆EBV DNA检测可作为评价鼻咽癌转移患者化疗疗效的一项血清学指标,与影像学联合应用可提高鼻咽癌疗效评价的准确性。
Objective： The present study explores the significance of Epstein-Barr virus DNA （EBV-DNA） in the diagnosis and monitoring of the clinical responses of patients with metastatic nasopharyngeal carcinoma （NPC）. Methods： Plasma was collected from 16 metastatic NPC patients admitted to the Cancer Hospital of Zhongshan University between December 2006 and June 2007. All patients underwent six cycles of chemotherapy with the regimen of cisplatin plus 5-Fu and individual taxol/carboplatin. Peripheral blood was collected at every other two cycles of chemotherapy, and the response was assessed using radiological examination. A real-time quantitative polymerase chain reaction system was used to determine the Epstein-Barr virus DNA in the plasma. Results： After the chemotherapy, as the EBV-DNA concentration decreased, the variation in the level of plasma EBV-DNA reflected the tumor response, with a concordance rate over 80%. When the EBV-DNA concentration increased, the variation in the level of plasma EBV-DNA reflected the actual tumor response, with a concordance rate of 100%. When the EBV-DNA concentration was always 0, the tumor response was not reflected. After comparing the relationship between the short effect and the change in the log EBV-DNA before and after chemotherapy, the means of the changes in the log EBV-DNA were -4.63, -2.25, and 3.23 in patients achieving partial remission, stable disease, and progression of disease, respectively. A statistical significance was obtained when the three groups were compared （P=0.025）. Conclusion： Plasma EBV-DNA could be a seto-diagnostic index in assessing the curative effect of metastatic NPC. Combined application of serology with radiography could improve the accuracy of the assessment.
出处
《中国肿瘤临床》
CAS
CSCD
北大核心
2012年第24期2005-2008,共4页
Chinese Journal of Clinical Oncology
关键词
转移鼻咽癌
EB病毒DNA
疗效评价
Metastatic nasopharyngeal carcinoma
Epstein-Barr virus DNA
Evaluation
