摘要
目的研究5型腺病毒（Ad5）不同途径感染小鼠后体内代谢和组织分布特点，为Ad5载体疫苗的应用和改造提供参考。方法构建携带萤火虫荧光素酶基因的Ad5-Fluc病毒并利用活体成像技术分析其感染BALB／c小鼠后的蛋白表达和组织分布特点，以及与裸鼠的感染特点进行比较。结果Ad5-Fluc肌肉途径感染BALB／c小鼠后出现肝脾转移表达的特点，且肌肉途径表达周期最长（〉60d），表达水平最高（P〈0.01）。病毒感染裸鼠后分布特点与BALB／c小鼠一致但表达周期明显延长。另外，用Ad35的纤突蛋白替代Ad5的纤突蛋白后的5型腺病毒载体（Ad5F35）在保持相似表达效率的同时消除了肝嗜性的特点。结论肌肉途径可能为5型腺病毒载体疫苗理想的免疫方式，同时Ad5F35病毒是一种理想的替代性腺病毒疫苗载体。
Objective To study the in vivo expression and biodistribution of AdS-Flac （Adenovi- rus carrying firefly luciferase genes） in mice. Methods The recombinant AdS-Fluc virus was constructed and infected to BALB/c or nude mice through three different routes. The protein expression level, tissue dis- tribution and the characteristics of infection were analyzed by in vivo bioluminescence imaging technology. Results Compared to other two routes, the BALB/c mice infected through muscular route had the longest expression cycle （over 60 days） and the highest expression level,while the virus was transferred into the liv- er and spleen after infection. The nude mice had a significantly extended expression cycle than BALB/c mice. Moreover, the characteristic of liver tropism was eliminated after AdS F35 infection in mice, while main- tained similar expression efficiency. Conclusion Due to the highest expression efficiency, the muscular route would be the optimal route for AdS vector based vaccination. In addition, Ad5F35 virus could become an ideal alternative vaccine vector for eliminating the liver tropism.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2012年第11期925-929,共5页
Chinese Journal of Microbiology and Immunology
