摘要
目的:观察Runt相关转录因子(Runt-related transcription factor,RUNX)1、RUNX2、RUNX3在小鼠下颌第一磨牙不同发育阶段的表达情况,探讨其在Balb/c小鼠牙齿发育过程中的作用机制。方法:取Balb/c小鼠出生前19.5d和出生后0、6、14、28d的包含下颌第一磨牙胚或牙齿的下颌骨组织,分别进行HE染色和免疫组织化学方法观察牙齿发育中RUNX1、RUNX2、RUNX3的表达情况。结果:RUNX1在胚胎19.5d的小鼠牙胚中有表达,出生当天、出生后6、14、28d,RUNX1的表达递增。RUNX2在胚胎19.5d和出生当天牙胚中呈颗粒状表达;出生后6、14、28d均有表达,表达量在出生后6d呈现最低,然后又在14、28d时升高。RUNX3在胚胎19.5d和出生当天牙胚中基本无表达;出生后6d,RUNX3在牙本质中表达量最低,出生后14、28d时表达量逐渐升高。结论:RUNX1在成釉细胞分化、成牙本质细胞分化和牙本质成熟过程中起一定作用,RUNX2与牙本质的成熟过程最相关,RUNX3主要与后期牙本质的成熟和牙齿形态相关。
Objective：To observe the expression of three members in the Runt-related transcription factor （RUNX） 1, RUNX2, RUNX3 in mandibular first molars of mice at different stages prenatally and postnatally, and explore the mechanism of RUNX in tooth development. Methods：The mandibles of Balb/c mice containing mandibular first molars or their germs were isolated at embryonal 19. 5 day and postnatal 0,6,14,28 days respectively. The isolated tissues were processed by HE staining and immunohistochemistrical method to detect the expression of RUNX1 ,RUNX2,RUNX3 in tooth development. Results：RUNX1 was detected at El9.5 day in the germs and progressively increased at P 0,6,14,28 days. The expression of RUNX2 was granularly at El9.5 day and P0 day. The lowest point was detected at P6 day, and an uprising positive trend was showed at P14 day and 28 day. Expression of RUNX3 showed a rising trend in dentine at P6,14,28 days but negative at El9.5 day and PO day. Conclusions ：It can be speculated that RUNX1 participates in differentiation of ameloblasts and odontoblasts and maturation of dentine. RUNX2 is mostly correlated with the maturation of dentin. RUNX3 is related to maturation of dentine and formation of tooth shape in later period.
出处
《口腔生物医学》
2012年第4期193-196,共4页
Oral Biomedicine
基金
江苏省自然科学基金(BK2012528)
江苏高校优势学科建设工程资助项目
