摘要
为检测人抗猪胰岛细胞天然抗体的同种型 ,采取 18份胰岛素依赖型糖尿病 (IDDM)患者血清及 2 0份正常人血清分别与杂种猪胰腺组织的冰冻切片孵化后 ,再用免疫荧光标记的羊抗人 Ig G、Ig M单克隆抗体染色。 8份 IDDM血清和 8份正常人血清中含有直接针对猪胰岛细胞的 Ig G型抗体 ;4份 IDDM血清和 6份正常人血清中含有 Ig M型抗猪胰岛细胞抗体。结果表明 :人抗猪胰岛细胞天然抗体的同种型是 Ig G和 Ig
To characterize the isotype of human xenoreactive natural antibodies against porcine pancrease islet cells, cyostat sections of the pancrease were incubated with the sera from 18 cases of IDDM or 20 human controls respectively, further with FITC conjugated goat antihuman IgG or IgM secondary antibodies. IgG was found to bind to pancrease islet cells in sera from 8 cases of IDDM and 8 human controls, while IgM in 4 IDDM and 6 human controls. It was suggested that isotypes of human xenoreative natural antibodies against porcine pancrease islet cells were IgG and IgM.
出处
《同济医科大学学报》
CSCD
2000年第3期232-233,共2页
Acta Universitatis Medicinae Tongji
基金
国家自然科学基金资助项目!(No.3970702)
关键词
猪
胰岛
天然抗体
同种型
swine
islands of langerhans
xenoreactive natural antibody
