摘要
为研究糜酶的功能，本文利用蛋白酶抑制剂Lisinopril、Aprotinin及EDTA结合放射免疫分析的方法同时测定了糜酶转基因小鼠心脏组织中血管紧张素转换酶（angiotensinconvertingenzyme，ACE）及糜酶样活性。结果显示转基因小鼠心脏组织中康酶比活力（0．274±0．071U／mg蛋白）较对照（0.152±0.021U／mg蛋白）显著升高，而ACE比活力不变（分别为0.172±0．029U／mg蛋白和0．177±0．019U／mg蛋白）。同时测定出转基因小鼠心脏局部血管紧张素Ⅱ（angiotensinⅡ，AngⅡ）含量（1984±184pg／g心脏组织）为对照（568±88pg／g心脏组织）的3倍。表明糜酶转基因小鼠心脏中可表达糜酶且糜酶能催化AngⅡ的生成，而蛋白酶抑制剂结合放免分析是一种快速、灵敏的检测ACE及糜酶样活性的方法。
To study the function of chymase, the protease inhibitors including Lisinopril, Aprotinin and EDTA, andradioimmunuoassy were used to measure the activities of angiotensin converting enzyme (ACE) and chymase in theheart tissues of transgenic mice harboring the human heart chymase gene and the controls. Compared to the control,it was showed that the chymase-like activity increased remarkably (0.274 ±0.071 U/mg protein vs 0. 52±0.021U/mg protein) but the activity of ACE unchanged (0.172±0.029U/mg protein vs 0. 177 ±0.019U/mg protein). Thelevel of angiotensin Ⅱ(Ang Ⅱ ) in the transgenic mice (1984 ±184pg/g heart tissue) were also measured as much asthree times than that in control (568 ± 88pg/g heart tissue) by radioimmunuoassy. The results suggested that thechymase could convert Ang Ⅰto Ang Ⅱin vivo, and the radioimmunoassay method could detect the activities of ACEand chymase simultaneously with high sensitivity and credibility.
出处
《基础医学与临床》
CSCD
2000年第3期90-92,共3页
Basic and Clinical Medicine
基金
国家自然科学基金!39570297
关键词
血管紧张素转换酶
糜酶
蛋白酶抑制剂
angiotensin converting enzyme
chymase
protease inhibitor
radioimmunoassay
