摘要
为优化微波辅助提取枳椇子总黄酮的工艺条件,采用L9(34)正交实验,考察指标为总黄酮提取率,以枳椇子浸泡时间、微波辐射功率、微波作用时间和乙醇浓度为四因素,比较了传统热回流提取和微波辅助提取两种方法对枳椇子总黄酮的提取效率和工艺参数.结果表明:影响微波提取效果的因素顺序为乙醇质量分数>微波辐射功率>微波作用时间>浸泡时间,最佳微波辅助提取枳椇子总黄酮工艺条件为浸泡时间30min,提取功率400W,微波提取时间15min,乙醇质量分数50%,在此条件下,总黄酮得率为2.18%.与传统的热回流提取法相比,微波辅助提取法提取效果接近,节约了溶剂,显著缩短了时间.微波辅助提取枳椇子总黄酮是一种快捷,有效并且绿色的提取方法,对枳椇子的进一步开发应用具有一定的指导意义.
Hovenia With th To optimize the conditions for microwave-assisted （MAE） of total flavonoids from seeds of acerba Lindl. , L9 （34） orthogonal experiments were applied to optimize extraction process. e extraction content of total flavonoids as evaluation index, influences of soaking time, microwave power, microwave extraction time, ethanol concentration on the extraction were investigated. The efficiency and extraction parameters of conventional heat-reflux extraction, microwave assisted extraction were comparatively determined. The results indicate that the order of the influences effecting the microwave extraction was the ethanol concentration〉the microwave power〉the microwave extraction time〉the soaking time. The optimum extraction parameters of microwave assisted extraction of flavonoids from seeds of Hovenia acerba Lindl. are described as follows, soaking time is 30 min, microwave power is 400 W, microwave extraction time is 15 min and ethanol concentration is 50%. Under the above conditions, the yield of flavonoids from seeds of Hovenia acerba Lindl. could reach to 2. 18%. Compared with the conventional heat-reflux extraction, the microwave- assisted extraction exhibits similar efficiency, fewer reagent and shoter extraction time. The procedure of the extraction is a rapid, efficient and green extracion technique and it can be helpful to further development of seeds of Hovenia acerba Lindl. dulcis Thunb.
出处
《武汉工程大学学报》
CAS
2012年第12期13-15,20,共4页
Journal of Wuhan Institute of Technology
基金
武汉工程大学研究生教育创新基金(2011-17)
关键词
微波辅助提取
总黄酮
枳椇子
microwave-assisted extraction
total flavonoid
Hovenia acerba Lindl.
