摘要
目的 探讨华蟾素注射液的镇痛作用及其机制。方法 采用热板法和机械压力法测定SD大鼠后爪缩爪反应潜伏期 (HWL)作为痛阈指标 ,观察腹腔注射 (IP)华蟾素的镇痛作用和纳洛酮对华蟾素增加的HWL的影响。 结果 热板法 :IP 1.0ml华蟾素大鼠双侧HWL明显增加(P <0 .0 0 1) ;IP 0 .5ml华蟾素大鼠右侧HWL明显增加 (P <0 .0 5) ,而左侧HWL无明显差异(P >0 .0 5)。机械压力法 :IP 1.0ml华蟾素大鼠右侧HWL明显增加 (P <0 .0 5) ,而左侧HWL无显著性差异 (P >0 .0 5)。IP 0 .5ml华蟾素大鼠双侧HWL均无显著性差异 (P >0 .0 5)。IP 0 .2mg纳洛酮能明显地拮抗华蟾素的镇痛作用 (P <0 .0 0 1)。结论 华蟾素具有镇痛作用 ;
Objective To investigate the analgesic effect of Cinobufacini injectio and its mechanism.Methods The hindpaw withdrawal latency (HWL) of SD rats on the hot-plate and the Randall Selitto test were uesd to measure the pain threshold norm,and the analgesic effect of Cinobufacini injection by intraperitoneal injection and the effect of naloxone on increased HWL were observed.Results hot-plate test:There was significant increases in bilateral HWL in the group IP 1.0 ml of cinobufacini (P<0.001). In the groups received 0.5 ml of cinobufacini, there was significant increase in right HWL (P<0.05), but there was no significant increase in left HWL(P>0.05) compared with the control group. The Randall Selitto Test:In the group received 1.0 ml of cinobufacini there was significant increases in right HWL ( P<0.05),but there was no significant increase in left HWL (P>0.05).There were no significant increase in bilateral HWL IP 0.5 ml of cinobufacini ( P>0.05) compared with the control group. The analgesic effect of cinobufacini could be obviously antagonized by IP 0.2 mg of naloxone (P<0.001).Conclusion The results suggest that the cinobufacini could enhance the analgesic effect in rats, and that both cinobufacini and opioid exist a specific synergistical interaction in the transmission of presumed nociceptive information in nervous system.
出处
《临沂医学专科学校学报》
2000年第2期81-84,共4页
Journal of Linyi Medical College
关键词
华蟾素注射液
镇痛作用
Cinobufacini
Analgesic effect
Naloxone
Hindpaw withdrawal latency (HWL)
