摘要
目的:建立白洋淀芦根药材的UPLC含量测定方法。方法:对10批白洋淀产的芦根药材采用70%甲醇为溶剂超声提取制备溶液,应用WATERS UPLC超高效液相色谱仪,BEH C18色谱柱,PDA检测器进行测定。以乙腈-水为流动相进行梯度洗脱,流速0.1 mL·min-1,柱温25℃,检测波长320 nm。结果:阿魏酸在0.038～0.38μg呈良好的线性关系(R2=0.9999)。白洋淀产芦根中阿魏酸的含量在0.051%～0.169%。结论:建立的白洋淀芦根药材中阿魏酸的UPLC含量测定方法快速、高效,为白洋淀芦根药材的质量控制提供了有效手段。
Objective： To establish UPLC determination method of Phragmitis Rhizoma in Baiyangdian Method： Ten samples of Phragmitis Rhizoma in Baiyangdian from different place were extracted by 70% methanol with ultrasonic extractor. Sample solutions were determined by Waters UPLC equipped with BEH C18 column and PDA detector, gradient eluted with acetonitrile-water as mobile phase. The flow rate was set to 0. 1 mL ·min^-1, while the column temperature was set at 25 ℃ , and the wavelength for detection was set to 320 nm. Result： The standard curve was in good linearity over the range of 0. 038-0. 38 μg （R^2 = 0. 999 9）. Conclusion ： The method established is simple, accurate and can be used to control the quality of Pharagmitis Rhizoma producted in Baiyangdian.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第1期102-105,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
河北省中医药管理局科研计划项目(2008069)
