摘要
目的探讨卵巢癌患者外周血Foxp3＋调节性T细胞（Treg细胞）的变化。方法分别采用流式细胞术、实时定量反转录聚合酶链反应和酶联免疫吸附测定法检测46例卵巢癌患者（卵巢癌组）和46例健康体检者（对照组）外周血CD4＋ Foxp3＋ Treg细胞百分率、Foxp3 mRNA表达水平以及血浆转化生长因子-β1（TGF-β1）水平，并进行比较。结果卵巢癌组CD4＋ Foxp3＋ Treg细胞百分率[（11．42±2．67）％]、Foxp3 mRNA表达水平（0．59±0．21）以及血浆TGF-β1水平[（35580±7274）ng／L]与对照组[分别为（8．94±1．98）％、0．37±0．14、（28610±5631）ng／L]相比显著增高，差异有统计学意义（P=0．0000）。结论卵巢癌患者外周血CD4＋ Foxp3＋ Treg细胞数量和／或功能异常，可能与卵巢癌的发生有关。
Objective To investigate the changes of peripheral blood Foxp3＋regulatory T cell （Treg cell） in patients with ovarian cancer （OC）. Methods In 46 patients with OC and 46 normal controls, the percentage of peripheral blood CD4＋Foxp3＋ Treg cell was assessed by flow cytometry and Foxp3 mRNA level was detected by real-time quantitative reverse transcription polymerase chain reaction. The level of plasma transforming growth factor β1 （TGF- β1） was measured by enzyme linked immunosorbent assay. Results The percentages of CD4＋Foxp3＋Treg cell, Foxp3 mRNA and level of plasma TGF-β 1 in patients with OC were statistically higher than those in normal controls [ （ 11.42 ± 2.67）% vs. （8.94 ± 1.98）%, 0.59 ± 0.21 vs. 0.37 ± 0.14, （35 580 ± 7274） ng/L vs. （28 610 ± 5631） ng/L,P = 0.0000]. Conclusion The number and/or function of CD4＋Foxp3＋Treg ceil in peripheral blood of patients with OC are abnormal, CD4＋Foxp3＋Treg cell may participate in the occurrence of OC.
出处
《中国医师进修杂志》
2012年第36期26-28,共3页
Chinese Journal of Postgraduates of Medicine
关键词
卵巢肿瘤
T淋巴细胞
调节性
转化生长因子Β1
Ovarian neoplasms
T-lymphocytes, regulatory
Transforming growth factor betal
