摘要
目的 观察维拉帕米 (verapamil)在半乳糖性白内障形成和发展过程中的作用。方法 5 5只Wistar雄性大鼠分为 4组 ,2组动物每日腹腔注射 5 0 %D 半乳糖两次 ,剂量为 30ml/ (kg·d) ,其中 1组每天皮下注射维拉帕米 12mg/ (kg·d) ,共 30天。另 2组注射相同剂量的生理盐水 ,其中 1组动物也给予皮下注射维拉帕米 ,观察时间相同。术后不同时间散瞳观察晶状体的动态变化 ,用日本岛津AA 6 40 13型原子吸收分光光度计检测血清和晶状体内的Na+ ,K+ ,Ca2 + 浓度。结果 半乳糖注射 30天后 ,未经治疗组几乎所有动物形成核性或核性以上的白内障 ,而治疗组所有动物晶状体均未形成核性混浊。维拉帕米能明显减轻白内障晶状体中Ca2 + ,K+ ,Na+ 浓度和Na+ /K+ 的变化。结论 维拉帕米能有效阻止半乳糖性白内障的形成和发展。
ObjectiveTo observe the effects on cataractogenesis of Ca 2+ antagonist drug verapamil to the rats of galactose induced cataract.MethodsFifty five 4 week old male Wistar rats were ascertained to have clear lens with slit lamp microscope under mydriasis.The animals were randomized into four groups.Two groups were administered 50% D galactose twice a day intraperitoneally for 30 d and one of the two groups also received verapamil 12 mg/(kg.d).The other two control groups were injected 0 9% saline twice a day intraperitoneally for the same days and verapamil at the same doses respectively.The lenses were monitored regularly under 1% atropine mydriasis with the slit lamp and the levels of Ca 2+ ,Na +,K + in sera and lens were determined by a Shimadzu AA 640[KG-6]-[KG-6]13 atomic absorption spectrophotometer.ResultsAlmost all lenses in galactose alone group formed nuclear opacity after 30 d,while all lenses in verapamil treated galactose group exhibited less opacity than a nuclear cataract.Lenticular electrolyte imbalance,particularly Ca 2+ ,in the injection galactose animals was closely correlated with cataract development,and verapamil significantly reduced the alteration in ion concentrations of Ca 2+ ,Na +,K +.Verapamil also decreased Na +/K + ratios of the galactose groups.ConclusionVerapamil could reduce the formation and development of galactose cataract.
出处
《眼科研究》
CSCD
2000年第2期117-119,共3页
Chinese Ophthalmic Research
