摘要
建立了生咖啡中赭曲霉毒素A的超高效液相色谱-串联质谱(UPLC-MS/MS)检测方法。样品用甲醇提取后,直接在BEH C18色谱柱上以乙腈-0.1%甲酸水为流动相分离,采用多离子监测模式(MRM)进行测定。赭曲霉毒素A的线性范围为5.0～200.0 ng/mL,相关系数大于0.99,检出限为0.5μg/kg。4个水平的添加回收率在84.3%～109.2%,相对标准偏差小于7.35%。本方法灵敏度高、准确性好、成本低,适用于生咖啡中赭曲霉毒素A的检测。
An analytical method was developed for the determination of ochratoxin A in green coffee by using ultra performance liquid chromatography coupled with tandem mass spectrometry （UPLC-MS/MS）. OTA was extracted by methanol, separated through BEH C18 column using acetonitrile/water （containing 0.1% formic acid） as mobile phase, and then quantified with multiple reactions monitoring （MRM）. Calibration curves exhibited good linearity over the concentration range from 5.0 to 200.0 ng/mL, with the correlation coefficients above 0.99. The limit of detection （LOD） for OTA was 0.5 izg/kg. Average recovery for OTA spiked at 4 levels ranged from 84.3% to 109.2%, with a relative standard deviations （RSD） less than 7.35%. The developed method was sensitive, accurate, low-cost, and can be applied for determination of OTA in green coffee.
出处
《热带农业科学》
2012年第12期87-90,共4页
Chinese Journal of Tropical Agriculture
基金
公益性行业(农业)科研专项(No.200903024-03)
