摘要
为利用生物降解草甘膦修复污染土壤,以多年施用草甘膦除草剂的农田土壤为材料,采用富集培养及逐级驯化方法,对长期施用草甘膦的农田土壤中分离得到1株草甘膦高效降解菌进行了研究。结果显示:被分离出的菌株HX-5,能以草甘膦为唯一碳源和氮源生长,对草甘膦的最高耐受浓度为4g/L。形态观察及生理生化特征研究,鉴定为节杆菌属(Arthrobacter sp.)。通过单因素试验确定了HX-5菌株生长的最适温度为30℃、最适pH 7.0及无机盐培养基中添加草甘膦的最适起始浓度为1.2g/L,在此条件下培养6d,草甘膦的降解率达74.83%。
In order to renovate contaminated soil through biodegradation, the degrading bacterium strain HX-5, which is capable of using glyphosate as sole carbon and nitrogen source, was isolated and purified from the field soil which have been applied with herbicide glyphosate for many years by enrichment culture and domestication successively techniques. The maximum concentration of glyphosate tolerant for strain HX-5 was 4.0 g/L. Based on the morphological observation, cultural and biochemical characteristics, HX-5 had been identified as Arthrobacter sp. The single-factor experiment showed that the optimum growth conditions of strain HX-5 included pH 7. 0, temperature 30℃ and glyphosate initial concentration 1. 2 g/L. The degradation rate reached 74. 83% after 6 days culturing under these conditions.
出处
《贵州农业科学》
CAS
北大核心
2012年第12期139-142,145,共5页
Guizhou Agricultural Sciences
基金
农业部转基因生物新品种培育重大专项子课题"转基因生物的‘基因删除’技术和‘基因拆分’技术的研究"(2011ZX08010-003)
关键词
草甘膦降解菌
分离鉴定
生长特性
降解率
glyphosate-degrading bacterium
isolation and identification
growth characteristics
degradation rate
