摘要
目的:采用免疫组化(VEGF)技术,观察鼻咽癌气血凝结型可能相关的分子水平微观表达,探讨其及对鼻咽癌放疗敏感性、复发和转移的影响,寻找一种有助于判断鼻咽癌放疗敏感性及其预后评价的微观指标。方法:将符合鼻咽癌气血凝结型的患者随机分为2组,试验组21例以祛瘀散结法为主的丹栀逍遥散加减口服治疗;对照组21例单纯进行放射治疗。取鼻咽肿瘤组织进行VEGF检测,同时利用MRI观察肿瘤放疗后的面积变化以评价放疗敏感性。结果:放疗后肿瘤缩小面积试验组大于对照组,差异有显著性意义(P<0.05);放疗后试验组VEGF水平较放疗前降低,差异非常有显著性意义(P<0.01);放疗后试验组全血黏度、血浆黏度、血沉方程K值、红细胞聚集指数、红细胞变形指数、血沉等均有降低,与放疗前比较,差异均有显著性意义(P<0.05);放疗后试验组血浆黏度、血沉方程K值、红细胞聚集指数、血沉等与对照组同期比较有明显降低,以上各组数据比较,差异均有显著性意义(P<0.05)。结论:VEGF可作为鼻咽癌气血凝结型相关的细胞、分子水平微观表达的指标,对放疗敏感性、复发和转移有一定的指导作用,为运用祛瘀散结法改善患者血液的高凝、高黏状态,提高放疗敏感性、防止复发及转移提供理论依据,并为鼻咽癌放疗中及放疗后有效中药新药开发及临床研究提供基础数据。
Objective： To observe the expression of vascular endothelial growth factor（VEGF） in na- sopharyngeal carcinoma patients with Qi-blood stagnation, and to evaluate the synergistic action of sta- sis-dispelling and mass-subduing therapy with radiotherapy. Methods： Forty-two nasopharyngeal carci- noma patients with Qi-blood stagnation were equally randomized into two groups. The treatment group was given radiotherapy and modified Danzhi Xiaoyao Powder orally which had the actions of dispelling stasis and subduing mass, and the control group was given radiotherapy. VEGF expression level in the tumor tissue was detected by immunohistochemical assay, and MRI examination was carried out for the evalua- tion of radiotherapy sensitivity. Results： The reduciton of tumor mass in the treatment group was more obvious than that in the control group after radiotherapy（P〈 0.05）. VEGF level was decreased in the treat- ment group（P〈 0.01 compared with that before treatment）, and whole blood viscosity, plasma viscosity, erythrosedimentation equation K value, erythrocyte aggregation, erythrosedimentation in the treatment group differed from those in the control gorup after treatment （P〈0.05）. Conclusion： VEGF expression level can be used as an index for the evaluation of radiotherapy sensitivity, tumor recurrence and metastasis in nasopharyngeal carcinoma patients with Qi-blood stagnation, and the stasis-dispelling and mass-subduing therapy can enhance the therapeutic effect of radiotherapy.
出处
《新中医》
CAS
2013年第1期60-63,共4页
New Chinese Medicine
基金
广东省中医药局2008年科研基金项目(编号:2008331)
关键词
鼻咽癌
中医证候
气血凝结
痰浊结聚
免疫组化(VEGF)
血液流变学
Nasopharyngeal carcinoma
Traditional Chinese Medical syndromes
Qi-blood stagna- tion
Phlegm-turbidity accumulation
VEGF
Blood rheology
