摘要
目的 :本研究检测了过氧化物酶系统 (乳过氧化物酶LP ,过氧化氢H2 O2 ,硫氰酸盐SCN-)及其各组份对变链菌葡糖基转移酶D(GtfD)的作用。方法 :变链菌GtfD由重组菌提纯 ,GtfD与含葡聚糖和蔗糖 (含C14 标记葡萄糖 )的氯化钾缓冲液孵育 ,经闪烁计数器测定GtfD活性 ;过氧化物酶系统及其组份对GtfD活性的影响 ,通过将这些组份单独或共同和GtfD孵育得以测定。结果和结论 :过氧化物酶系统抗微生物因子低硫氰酸 /低硫氰酸盐离子 (HOSCN/OSCN)对变形链球菌葡糖基转移酶D没有抑制作用 ,而乳过氧化物酶本身则抑制GtfD活性。然而 ,如果LP与其底物共同作用 ,则增加GtfD活性。
Objective: To investigate the effects of the lactoperoxidase system (LP, H 2O 2 , SCN -) and its components on the activity of glucosyltransferase D (GtfD) originating from S. mutans. Methods: GtfD of S. mutans was purified from recombinant bacteria. GtfD was incubated in buffered-KCl solution with dextran and sucrose containing sucrose- [ 14 C-glucose]. The bound activity of GtfD was measured with a scintillation counter. The effects of peroxidase system and its components on the activity of GtfD were examined by incubating different components separately and together with GtfD. Results and Conclusions: Lactoperoxidase system-generated antimicrobial hypothiocyanite acid/ions ( HOSCN/OSCN) had no effect on the activity of streptococcal GtfD while the lactoperoxidase enzyme as such inhibited GtfD. However, if LP was combined with its substrates, it enhanced the activity of GtfD. [
出处
《口腔医学纵横》
CAS
CSCD
2000年第2期87-89,共3页
Journal of Comprehensive Stomatology
基金
芬兰科学院
芬兰牙科学协会和中国留学基金委资助
