摘要
目的了解温州地区临床分离的金黄色葡萄球菌(SA)的耐药特点,探讨SA中耐β-内酰胺类、氨基糖苷类、四环素类药物耐药基因及耐消毒剂基因(qacA)的存在情况。方法采用聚合酶链式反应(PCR)法对SA进行β-内酰胺酶基因、氨基糖苷类修饰酶基因、四环素类基因和耐消毒剂基因检测。结果 PCR结果显示94株SA中耐药相关基因检出率mecA 53.2%、aac(6')/aph(2″)68.1%、aph(3')-III 37.2%、tetM 53.2%和qacA 7.4%,其中59株MRSA的耐药相关基因检出率分别为mecA 83.1%、aac(6')/aph(2″)86.4%、aph(3')-III 42.4%、tetM 76.4%和qacA 8.5%。结论多数SA菌株存在耐β-内酰胺类、氨基糖苷类、四环素类等多种抗生素耐药基因,具有多重耐药特征,但尚未出现明显耐消毒剂状况。
Objective To study the drug resistance genes and disinfectant-resistant genes of clinical Staphylococcus aureus isolates.Methods Common drug-resistant genes associated with β-lactamases,aminoglycosides,tetracyclines and disinfectants were detected by PCR.Results The positive rates of drug resistant genes in 94 SA strains were 53.2% for mecA,68.1% for aac（6′）/aph（2″）,37.2% for aph（3′）-III,53.2% for tetM and 7.4% for qacA.The positive rates of drug resistant genes in 59 MRSA strains were 83.1% for mecA,86.4% for aac（6′）/aph（2″）,42.4% for aph（3′）-III,76.4% for tetM and 8.5% for qacA.Conclusion There are resistance genes of β-lactams,aminoglycosides,and tetracycline in most of the SA strains,but the disinfectants-resistant genes are not prevalent.
出处
《中国微生态学杂志》
CAS
CSCD
2012年第12期1103-1105,共3页
Chinese Journal of Microecology
基金
温州市科技局项目(Y20090382)
关键词
金黄色葡萄球菌
耐药基因
耐消毒剂基因
Staphylococcus aureus
Drug-resistant gene
Disinfectant-resistant gene
