摘要
目的探讨细胞凋亡抑制因子bcl-2、细胞凋亡促进因子bax、ER蛋白在子宫肌层不同浸润深度的子宫腺肌病（腺肌病）病灶腺上皮细胞中的表达变化及意义。方法选择2009年9月至2010年9月在北京安贞医院住院手术，并经病理检查证实为腺肌病的患者36例（合并盆腔内异症、卵巢子宫内膜异位囊肿者除外）为腺肌病组，采用免疫组化SP法，检测其子宫浅肌层（肌层浸润深度〈1／2）和深肌层（肌层浸润深度≥1／2）异位内膜、在位子宫内膜组织及同期因卵巢浆液性囊腺瘤、卵巢冠囊肿、子宫中隔以及盆腔器官脱垂行手术治疗的30例患者（对照组）的正常子宫内膜组织中bcl-2、bax、ER蛋白的表达情况及其相关性。结果（1）ER蛋白的表达：腺肌病组浅肌层和深肌层异位内膜中ER蛋白表达水平（5．04±0．24、4．91±0．16）低于在位内膜（6．06±0．36），差异有统计学意义（P〈0．01），但高于对照组子宫内膜的3．70±0．58，差异也有统计学意义（P〈0．05）；但腺肌病组深、浅肌层异位内膜中ER蛋白表达水平比较，差异无统计学意义（P〉0．05）。（2）bcl-2蛋白的表达：腺肌病组bcl-2蛋白表达水平在浅肌层异位内膜中为5．6±0．4，深肌层异位内膜中为6．0±0．3，均高于在位内膜的3．6±0．4和对照组子宫内膜的1．9±0．4，差异均有统计学意义（P〈0．01）。（3）bax蛋白的表达：腺肌病组bax蛋白的表达水平在浅肌层异位内膜中为3．50±0．28，深肌层异位内膜中为4．80±0．29，在位内膜为4．43±0．37，均低于对照组子宫内膜的6．18±0．65，差异有统计学意义（P〈0．05），浅肌层异位内膜中bax蛋白的表达水平低于深肌层异位内膜，差异也有统计学意义（P〈0．01）。（4）ER与bcl-2、bax蛋白表达的相关性：腺肌病组浅肌层异位内膜中ER蛋白与bcl．2蛋白表达水平呈正相关（
Objective To investigate the expression and signification of B-cell lymphoma/ leukemia-2（ bel-2）, bax and ER proteins in epithelial cells in different depth of adenomyosis. Methods Expression and correlation of bcl-2, bax and ER proteins were detected by immunohistoehemistry staining in 36 adenomyosis cases with superficial （ 〈 1/2 depth of penetration） and deep （≥ 1/2 depth of penetration） ectopic endometrium and eutopic endometrial tissues matched with 30 cases with benign ovarian tumor, uterine septum, pelvic floor dysfunction without adenomyosis as controls. Results （ 1 ） The expression of ER protein in superficial and deep ectopic tissues （5.04 ± 0. 24,4. 91 ± 0. 16） were found significantly lower than 6.06 ± 0. 36 in eutopic tissues （ P 〈 0. 01 ） and higher than 3.70 ± 0. 58 in control group （P 〈 0. 05 ）.There was no statistical difference of ER expression in superficial and deep ectopic endometrium （ P 〉 0. 05 ）. （ 2 ） The level of bcl-2 protein of 5.6 ± 0. 4 in superficial and 6. 0 _± 0. 3 in deep myometrium of ectopic tissues were significantly higher than 3.6 ± 0. 4 in eutopic tissues and 1.9 ± 0. 4 in control group （P 〈0. 01 ）. （3） The level of bax protein of 3.50±0. 28 in superficial and 4. 80 ±0. 29 in deep myometrial ectopic and 4.43 ±0. 37 in eutopic tissues were significantly lower than 6. 18 ±0.65 in control groups （P 〈 0. 05）. The expression of bax in superficial myometrium is significantly lower than deep myometrium in ectopic tissues （P 〈0. 01 ）. （4） The positive correlation between the expression of ER and bcl-2 protein at superficial myometrium of ectopic tissues （r = 0. 720, P 〈 0. 01 ）. And there was no significant correlation with the expression of ER and bax protein at superficial myometrium （ r = 0. 008, P 〉 0. 05 ）. As well as, there was not significant correlation with the expression of bcl-2, bax and ER protein at deep myometrium （r = 0. 089, r = - 0. 023, P 〉 0. 05 ）. The expression
出处
《中华妇产科杂志》
CAS
CSCD
北大核心
2012年第12期923-927,共5页
Chinese Journal of Obstetrics and Gynecology
基金
首都医科大学基础-临床科研合作基金（09JL58）
